Rekombinanter NeuN Antikörper (AA 30-60)

Produktnummer ABIN7126261

Produktdetails anti-NeuN Antikörper

Target: NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))

Antikörpertyp: Recombinant Antibody

Bindungsspezifität: AA 30-60

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser NeuN Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: NeuN antibody specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuro. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function, however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: A synthetic peptide corresponding to residues within aa 30-60 of human NeuN protein (exact sequence is proprietary)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Brain tissue.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu NeuN

Target: NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))

Andere Bezeichnung: RBFOX3 (RBFOX3 Produkte)

Synonyme: FOX-3 antikoerper, FOX3 antikoerper, HRNBP3 antikoerper, NEUN antikoerper, Fox-3 antikoerper, Hrnbp3 antikoerper, NeuN antikoerper, Neuna60 antikoerper, Neun antikoerper, RGD1560070 antikoerper, RNA binding fox-1 homolog 3 antikoerper, RNA binding protein, fox-1 homolog (C. elegans) 3 antikoerper, RNA binding protein, fox-1 homolog 3 antikoerper, RBFOX3 antikoerper, Rbfox3 antikoerper

Hintergrund: FLJ56884, FLJ58356, fox1 homolog C, Fox3, FOX3NeuN, hexaribonucleotide binding protein 3 (HRNBP3), neuronal nuclei, Rbfox3, RNA binding protein, fox 1 homolog (C. elegans) 3,Neuronal-Nuclei (NeuN) (Neuronal Marker)
Cellular localisation: Nucleus. Cytoplasm.

Molekulargewicht: 38kDa

Gen-ID: 146713, 135229

UniProt: A6NFN3