TDRKH Antikörper

Produktnummer ABIN7126210

Dieses Anti-TDRKH-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von TDRKH in WB, IF, IP, FACS und IHC (f). Geeignet für Human.

Kurzübersicht für TDRKH Antikörper (ABIN7126210)

Target: TDRKH (Tudor and KH Domain Containing (TDRKH))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TDRKH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-TDRKH Antikörper

Spezifität: Participates in the primary piRNA biogenesis pathway and is required during spermatogenesis to repress transposable elements and prevent their mobilization, which is essential for the germline integrity. The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Required for the final steps of primary piRNA biogenesis by participating in the processing of 31-37 nt intermediates into mature piRNAs. May act in pi-bodies and piP-bodies by transferring piRNA precursors or intermediates to or between these granules.

Kreuzreaktivität (Details): Human. Predicted to react with Mouse and Rat.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human TDRKH protein

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or MCF7 cells. Human testis or brain.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu TDRKH

Target: TDRKH (Tudor and KH Domain Containing (TDRKH))

Andere Bezeichnung: TDRKH

Hintergrund: 2700091C21Rik, Putative RNA binding protein, RGD1311196, RP11-98D18.8, TDRD2, TDRD7, Tdrkh, Tudor and KH domain containing, Tudor and KH domain-containing protein, tudor domain containing 2,TDRKH
Cellular localisation: Cytoplasm. Localized to cytosol and centrosome.

Molekulargewicht: 62.05kDa

Gen-ID: 11022, 144439

UniProt: Q9Y2W6