Periostin Antikörper (AA 193-326)

Produktnummer ABIN7504148

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Periostin in IHC (f). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Periostin Antikörper (AA 193-326) (ABIN7504148)

Target: Periostin (POSTN)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Periostin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-Periostin Antikörper

Bindungsspezifität: AA 193-326

Spezifität: Periostin (PN), also designated osteoblast-specific factor 2 (OSF-2), is a disulfide linked protein originally isolated as a osteoblast-specific factor. Periostin is a secreted protein that binds heparin and functions as a ligand for i±Vi²3 and i±Vi²5 integrins. In preosteoblasts, Periostin acts as a cell adhesion molecule and plays a role in osteoblast recruitment, spreading and attachment. Periostin is mainly detected in lower gastrointestinal tract, aorta, stomach, placenta, uterus and breast tissues but is up-regulated in epithelial ovarian tumors and overexpressed in breast cancer. Expression of Periostin is increased by bone morphogenetic protein (BMP2) and transforming growth factor i² 1 (TGF i² 1). Periostin contains a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain, which shows homology with the insect protein fasciclin, and a C-terminal domain.

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa193-326) of human POSTN protein (exact sequence is proprietary)

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Positive Control: Human placenta, breast or colon tissue.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Haltbarkeit: 24 months

Details zu Periostin

Target: Periostin (POSTN)

Andere Bezeichnung: POSTN

Hintergrund: Fasciclin-I like, Osteoblast specific factor 2 (OSF2), PDLPOSTN, Periodontal ligament specific periostin, Periostin isoform thy2 / th4 / thy6 / thy8, Periostin osteoblast specific factor, PN, POSTN,Periostin (POSTN)
Cellular localisation: Secreted > extracellular space > extracellular matrix.

Molekulargewicht: 84/74kDa (Periostin), 90kDa (secreted glycoprotein)

Gen-ID: 10631, 136348, 721018

UniProt: Q15063