SMNDC1 Antikörper

Produktnummer ABIN7504151

Produktdetails anti-SMNDC1 Antikörper

Target: SMNDC1 (Survival Motor Neuron Domain Containing 1 (SMNDC1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SMNDC1 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Spezifität: SPF30 (survival of motor neuron-related-splicing factor 30), also known as SMNDC1 (survival motor neuron domain containing 1) or SMNR (SMN-related protein), is an essential splicing factor required for spliceosome assembly that belongs to the SMN family. It contains one Tudor domain with significant similarity to SMN (survival motor neuron) and is expressed in skeletal muscle, pancreas and heart, localizing to Cajal bodies and nuclear speckles. SPF30 plays an important role in spliceosome assembly and directly interacts with five U snRNPs. The loss of SPF30 causes spliceosome assembly to arrest at prespliceosomes (A complex). This supports a function for SPF30 in mediating the incorporation/recruitment of U4/U5/U6 tri-snRNP to the prespliceosome. In addition, the overexpression of SPF30 can lead to apoptosis.

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human SMNDC1 protein

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa or HepG2 cells.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Haltbarkeit: 24 months

Details zu SMNDC1

Target: SMNDC1 (Survival Motor Neuron Domain Containing 1 (SMNDC1))

Andere Bezeichnung: SMNDC1 (SMNDC1 Produkte)

Hintergrund: 30 kDa splicing factor SMNrp, MGC106917, MGC112663, SMN related protein, SMNR, SPF30, Splicing factor 30, survival of motor neuron-related, Survival motor neuron domain containing protein 1, Survival of motor neuron related splicing factor 30,SMNDC1
Cellular localisation: Nucleus. Detected in nuclear speckles containing snRNP and in Cajal (coiled) bodies.

Molekulargewicht: 30kDa

Gen-ID: 10285, 632093

UniProt: O75940