Mesothelin Antikörper (AA 273-407) (Biotin)

Produktnummer ABIN7126105

Dieses Anti-Mesothelin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Mesothelin in IHC (f). Geeignet für Human.

Kurzübersicht für Mesothelin Antikörper (AA 273-407) (Biotin) (ABIN7126105)

Target: Mesothelin (MSLN)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Mesothelin Antikörper ist konjugiert mit Biotin

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-Mesothelin Antikörper (Biotin)

Bindungsspezifität: AA 273-407

Spezifität: Mesothelin is a 40 kDa glycosyl-phosphatidylinositol-anchored glycoprotein cleaved from a 69 kDa precursor protein. Mesothelin immunoreactivity is high in cancers of the ovary (serous papillary, endometrioid and undifferentiated) and pancreas, with less frequent staining seen in adenocarcinomas of the endometrium, lung and stomach/esophagus. Mesothelin is one of the most sensitive markers for mesothelioma.

Kreuzreaktivität (Details): Human, Mouse and Rat.

Aufreinigung: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin.

Immunogen: Recombinant fragment (around aa 273-407) of human Mesothelin (MSLN) protein (exact sequence is proprietary)

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

Positive Control: CAPAN-2 or HeLa cells. Lung Mesothelioma.

Known Application: Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 100 μg/mL

Buffer: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Haltbarkeit: 24 months

Details zu Mesothelin

Target: Mesothelin (MSLN)

Andere Bezeichnung: MSLN

Hintergrund: CAK1, Megakaryocyte potentiating factor, Mesothelin, MSLN, SMR, SMRP,Mesothelin (Mesothelial Marker)
Cellular localisation: Cell Surface and Secreted

Molekulargewicht: 40kDa (Mature form), 69kDa (Precursor)

Gen-ID: 10232, 408488

UniProt: Q13421

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process