VPS35 Antikörper

Produktnummer ABIN7120708

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch VPS35 in WB, IP, IF und ELISA. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für VPS35 Antikörper (ABIN7120708)

Target: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser VPS35 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), ELISA

Produktdetails anti-VPS35 Antikörper

Aufreinigung: Immunogen affinity purified

Reinheit: ≥95 % as determined by SDS-PAGE

Immunogen: vacuolar protein sorting 35 homolog(S. cerevisiae)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:200-1:1000, IP: 1:200-1:1000, IF: 1:10-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Haltbarkeit: 12 months

Details zu VPS35

Target: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Andere Bezeichnung: VPS35

Hintergrund: Synonyms:DKFZp434E1211, DKFZp434P1672, FLJ10752, FLJ13588, FLJ20388, hVPS35, Maternal embryonic 3, MEM3, Vesicle protein sorting 35, VPS35 Background:Acts as component of the retromer cargo-selective complex(CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The CSC seems to associate with the cytoplasmic domain of cargo proteins predominantly via VPS35, however, these interactions seem to be of low affinity and retromer SNX proteins may also contribute to cargo selectivity thus questioning the classical function of the CSC. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network(TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5(Probable). Required for retrograde transport of lysosomal enzyme receptor IGF2R and SLC11A2. Required to regulate transcytosis of the polymeric immunoglobulin receptor(pIgR-pIgA)(PubMed:15078903, PubMed:15247922, PubMed:20164305). Required for endosomal localization of FAM21C(PubMed:22070227). Mediates the association of the CSC with the WASH complex via FAM21(PubMed:22070227, PubMed:24980502, PubMed:24819384). Required for the endosomal localization of TBC1D5(PubMed:20923837).

Molekulargewicht: 92 kDa

Gen-ID: 55737

UniProt: Q96QK1