Spt6 Antikörper

Produktnummer ABIN7119612

Dieses Anti-Spt6-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Spt6 in WB, IP und ELISA. Geeignet für Human, Maus und Ratte.

Kurzübersicht für Spt6 Antikörper (ABIN7119612)

Target: Spt6 (SUPT6H) (Suppressor of Ty 6 Homolog (SUPT6H))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Spt6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Produktdetails anti-Spt6 Antikörper

Aufreinigung: Immunogen affinity purified

Reinheit: ≥95 % as determined by SDS-PAGE

Immunogen: suppressor of Ty 6 homolog(S. cerevisiae)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:2000, IP: 1:200-1:2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Haltbarkeit: 12 months

Details zu Spt6

Target: Spt6 (SUPT6H) (Suppressor of Ty 6 Homolog (SUPT6H))

Andere Bezeichnung: SUPT6H

Hintergrund: Synonyms:KIAA0162, SPT6H Background:Transcription elongation factor which binds histone H3 and plays a key role in the regulation of transcription elongation and mRNA processing. Enhances the transcription elongation by RNA polymerase II(RNAPII) and is also required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. Besides chaperoning histones in transcription, acts to transport and splice mRNA by forming a complex with IWS1 and the C-terminal domain(CTD) of the RNAPII subunit RPB1(POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors(ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes(such as SETD2), to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription. SUPT6H via its association with SETD1A, regulates both class-switch recombination and somatic hypermutation through formation of H3K4me3 epigenetic marks on activation-induced cytidine deaminase(AICDA) target loci. Promotes the activation of the myogenic gene program by entailing erasure of the repressive H3K27me3 epigenetic mark through stabilization of the chromatin interaction of the H3K27 demethylase KDM6A.

Molekulargewicht: 230 kDa

Gen-ID: 9913

UniProt: Q7KZ85

Pathways: Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Production of Molecular Mediator of Immune Response