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RTF1 Antikörper

Der Rabbit Polyclonal Anti-RTF1-Antikörper wurde für WB, ELISA, IHC validiert. Er ist geeignet, RTF1 in Proben von Human, Mouse, Rat zu detektieren.
Produktnummer ABIN7118711

Kurzübersicht für RTF1 Antikörper (ABIN7118711)

Target

Alle RTF1 Antikörper anzeigen
RTF1 (RNA polymerase-associated protein RTF1 homolog (RTF1))

Reaktivität

  • 40
  • 5
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Human, Maus, Ratte

Wirt

  • 39
  • 1
Kaninchen

Klonalität

  • 40
Polyklonal

Konjugat

  • 17
  • 4
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser RTF1 Antikörper ist unkonjugiert

Applikation

  • 20
  • 16
  • 14
  • 13
  • 5
  • 4
  • 4
  • 4
  • 3
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
  • Aufreinigung

    Immunogen affinity purified

    Reinheit

    ≥95 % as determined by SDS-PAGE

    Immunogen

    Rtf1, Paf1/RNA polymerase II complex component, homolog(S. cerevisiae)

    Isotyp

    IgG
  • Applikationshinweise

    WB: 1:500-1:2000, IHC: 1:20-1:200

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Buffer

    PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

    Haltbarkeit

    12 months
  • Target

    RTF1 (RNA polymerase-associated protein RTF1 homolog (RTF1))

    Andere Bezeichnung

    RTF1

    Hintergrund

    Synonyms:KIAA0252 Background:Component of the PAF1 complex(PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'-and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1, it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4'(H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B(H2BK120ub1), UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Binds single-stranded DNA. Required for maximal induction of heat-shock genes. Required for the trimethylation of histone H3 'Lys-4'(H3K4me3) on genes involved in stem cell pluripotency, this function is synergistic with CXXC1 indicative for an involvement of a SET1 complex(By similarity).

    Molekulargewicht

    100 kDa

    Gen-ID

    23168

    UniProt

    Q92541

    Pathways

    Stem Cell Maintenance
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