PUM2 Antikörper

Produktnummer ABIN7118125

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PUM2 in WB und ELISA. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für PUM2 Antikörper (ABIN7118125)

Target: PUM2 (Pumilio Homolog 2 (Drosophila) (PUM2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PUM2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-PUM2 Antikörper

Aufreinigung: Immunogen affinity purified

Reinheit: ≥95 % as determined by SDS-PAGE

Immunogen: pumilio homolog 2(Drosophila)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Haltbarkeit: 12 months

Details zu PUM2

Target: PUM2 (Pumilio Homolog 2 (Drosophila) (PUM2))

Andere Bezeichnung: PUM2

Hintergrund: Synonyms:KIAA0235, PUMH2 Background:Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element(PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element(NRE)(, PubMed:21397187). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation(PubMed:22955276). Also mediates deadenylation-independent repression by promoting accessibility of miRNAs(PubMed:18776931, PubMed:22345517). Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation(PubMed:22345517). Plays a role in cytoplasmic sensing of viral infection(PubMed:25340845). Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD(non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm(PubMed:26724866). May regulate DCUN1D3 mRNA levels(PubMed:25349211). May support proliferation and self-renewal of stem cells.

Molekulargewicht: 140 kDa

Gen-ID: 23369

UniProt: Q8TB72

Pathways: Ribonucleoprotein Complex Subunit Organization