PNPT1 Antikörper

Produktnummer ABIN7117691

Der Kaninchen Polyklonal anti-PNPT1 Antikörper wird verwendet zum Nachweis von PNPT1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA, IHC und IP.

Kurzübersicht für PNPT1 Antikörper (ABIN7117691)

Target: PNPT1 (Polyribonucleotide Nucleotidyltransferase 1 (PNPT1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PNPT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Produktdetails anti-PNPT1 Antikörper

Verwendungszweck: PNPT1 antibody

Aufreinigung: Immunogen affinity purified

Reinheit: ≥95 % as determined by SDS-PAGE

Immunogen: polyribonucleotide nucleotidyltransferase 1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:2000, IP: 1:200-1:1000, IHC: 1:20-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze / thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C for 12 months

Haltbarkeit: 12 months

Details zu PNPT1

Target: PNPT1 (Polyribonucleotide Nucleotidyltransferase 1 (PNPT1))

Andere Bezeichnung: PNPT1

Hintergrund:

Synonyms: Polyribonucleotide nucleotidyltransferase 1, mitochondrial|3'-5' RNA exonuclease OLD35|PNPase old-35|Polynucleotide phosphorylase 1 (PNPase 1)|Polynucleotide phosphorylase-like protein|PNPT1|PNPASE

Background: RNA-binding protein implicated in numerous RNA metabolic processes. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'-to-5' direction. Mitochondrial intermembrane factor with RNA-processing exoribonulease activity. Component of the mitochondrial degradosome(mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Required for correct processing and polyadenylation of mitochondrial mRNAs. Plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components, like the 5S RNA, the RNA subunit of ribonuclease P and the mitochondrial RNA-processing(MRP) RNA, into the mitochondrial matrix. Plays a role in mitochondrial morphogenesis and respiration, regulates the expression of the electron transport chain(ETC) components at the mRNA and protein levels. In the cytoplasm, shows a 3'-to-5' exoribonuclease mediating mRNA degradation activity, degrades c-myc mRNA upon treatment with IFNB1/IFN-beta, resulting in a growth arrest in melanoma cells. Regulates the stability of specific mature miRNAs in melanoma cells, specifically and selectively degrades miR-221, preferentially. Plays also a role in RNA cell surveillance by cleaning up oxidized RNAs. Binds to the RNA subunit of ribonuclease P, MRP RNA and miR-221 microRNA.

Molekulargewicht: 90 kDa

Gen-ID: 87178

UniProt: Q8TCS8