POLR3A Antikörper (AA 1-200)

Produktnummer ABIN7117679

Der Kaninchen Polyklonal Anti-POLR3A-Antikörper wurde für ELISA und WB validiert. Er ist geeignet, POLR3A in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für POLR3A Antikörper (AA 1-200) (ABIN7117679)

Target: POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POLR3A Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB)

Produktdetails anti-POLR3A Antikörper

Bindungsspezifität: AA 1-200

Verwendungszweck: POLR3A antibody

Aufreinigung: Immunogen affinity purified

Reinheit: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 1-200aa

Immunogen: polymerase(RNA) III(DNA directed) polypeptide A, 155kDa

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze / thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C for 12 months

Haltbarkeit: 12 months

Details zu POLR3A

Target: POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))

Andere Bezeichnung: POLR3A

Hintergrund:

Synonyms: DNA-directed RNA polymerase III subunit RPC1 (RNA polymerase III subunit C1)|DNA-directed RNA polymerase III largest subunit|DNA-directed RNA polymerase III subunit A|RNA polymerase III 155 kDa subunit (RPC155)|RNA polymerase III subunit C160|POLR3A

Background: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition(By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs(EBERs) induce type I interferon and NF-Kappa-B through the RIG-I pathway.

Molekulargewicht: 140-150 kDa

Gen-ID: 11128

UniProt: O14802