ISCU Antikörper

Produktnummer ABIN7115516

Der Kaninchen Polyklonal anti-ISCU Antikörper wird verwendet zum Nachweis von ISCU in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA, IHC, IF und IP.

Kurzübersicht für ISCU Antikörper (ABIN7115516)

Target: ISCU (Iron-sulfur cluster assembly enzyme ISCU, mitochondrial (ISCU))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ISCU Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Produktdetails anti-ISCU Antikörper

Verwendungszweck: ISCU antibody

Aufreinigung: Immunogen affinity purified

Reinheit: ≥95 % as determined by SDS-PAGE

Immunogen: iron-sulfur cluster scaffold homolog(E. coli)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:2000, IP: 1:200-1:1000, IHC: 1:20-1:200, IF: 1:20-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze / thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C for 12 months

Haltbarkeit: 12 months

Details zu ISCU

Target: ISCU (Iron-sulfur cluster assembly enzyme ISCU, mitochondrial (ISCU))

Andere Bezeichnung: ISCU

Hintergrund:

Synonyms: Iron-sulfur cluster assembly enzyme ISCU|NifU-like N-terminal domain-containing protein|NifU-like protein|ISCU|NIFUN

Background: Scaffold protein for the de novo synthesis of iron-sulfur(Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins(PubMed:11060020). First, a [2Fe-2S] cluster is transiently assembled on the scaffold protein ISCU. In a second step, the cluster is released from ISCU, transferred to a glutaredoxin GLRX5, followed by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and their target-specific insertion into the recipient apoproteins. Cluster assembly on ISCU depends on the function of the cysteine desulfurase complex NFS1-LYRM4/ISD11, which serves as the sulfur donor for cluster synthesis, the iron-binding protein frataxin as the putative iron donor, and the electron transfer chain comprised of ferredoxin reductase and ferredoxin, which receive their electrons from NADH(By similarity).

Molekulargewicht: 14-15 kDa, 18-19 kDa

Gen-ID: 23479

UniProt: Q9H1K1