F-Box DNA Helicase 1 (FBXO18) Antikörper

Produktnummer ABIN7114057

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch in ELISA und WB. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für F-Box DNA Helicase 1 (FBXO18) Antikörper (ABIN7114057)

Target: F-Box DNA Helicase 1 (FBXO18)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: ELISA, Western Blotting (WB)

Produktdetails

Verwendungszweck: FBH1 antibody

Aufreinigung: Immunogen affinity purified

Reinheit: ≥95 % as determined by SDS-PAGE

Immunogen: F-box protein, helicase, 18

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze / thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C for 12 months

Haltbarkeit: 12 months

Antigendetails

Target: F-Box DNA Helicase 1 (FBXO18)

Andere Bezeichnung: FBH1

Hintergrund:

Synonyms: F-box DNA helicase 1 (hFBH1)|DNA 3'-5' helicase 1|F-box only protein 18|FBH1|FBX18|FBXO18

Background: 3'-5' DNA helicase and substrate-recognition component of the SCF(FBXO18) E3 ubiquitin ligase complex that plays a key role in response to stalled/damaged replication forks(PubMed:11956208, PubMed:23393192). Involved in genome maintenance by acting as an anti-recombinogenic helicase and preventing extensive strand exchange during homologous recombination: promotes RAD51 filament dissolution from stalled forks, thereby inhibiting homologous recombination and preventing excessive recombination(PubMed:17724085, PubMed:19736316). Also promotes cell death and DNA double-strand breakage in response to replication stress: together with MUS81, promotes the endonucleolytic DNA cleavage following prolonged replication stress via its helicase activity, possibly to eliminate cells with excessive replication stress(PubMed:23319600, PubMed:23361013). Plays a major role in remodeling of stalled DNA forks by catalyzing fork regression, in which the fork reverses and the two nascent DNA strands anneal(PubMed:25772361). In addition to the helicase activity, also acts as the substrate-recognition component of the SCF(FBXO18) E3 ubiquitin ligase complex, a complex that mediates ubiquitination of RAD51, leading to regulate RAD51 subcellular location(PubMed:25585578).

Molekulargewicht: 118-123 kDa

Gen-ID: 84893

UniProt: Q8NFZ0