DDX21 Antikörper

Produktnummer ABIN7113335

Der Kaninchen-Polyklonal Anti-DDX21-Antikörper wurde für WB, IHC, IF, ELISA und IP validiert. Er ist geeignet zum Nachweis von DDX21 in Proben aus Human, Maus und Ratte. }

Kurzübersicht für DDX21 Antikörper (ABIN7113335)

Target: DDX21 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 21 (DDX21))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DDX21 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunoprecipitation (IP)

Produktdetails anti-DDX21 Antikörper

Verwendungszweck: DDX21 antibody

Aufreinigung: Immunogen affinity purified

Reinheit: ≥95 % as determined by SDS-PAGE

Immunogen: DEAD(Asp-Glu-Ala-Asp) box polypeptide 21

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:2000, IP: 1:200-1:1000, IHC: 1:20-1:200, IF: 1:20-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze / thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C for 12 months

Haltbarkeit: 12 months

Details zu DDX21

Target: DDX21 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 21 (DDX21))

Andere Bezeichnung: DDX21

Hintergrund:

Synonyms: Nucleolar RNA helicase 2|DEAD box protein 21|Gu-alpha|Nucleolar RNA helicase Gu|Nucleolar RNA helicase II|RH II/Gu|DDX21

Background: RNA helicase that acts as a sensor of the transcriptional status of both RNA polymerase(Pol) I and II: promotes ribosomal RNA(rRNA) processing and transcription from polymerase II(Pol II)(PubMed:25470060). Binds various RNAs, such as rRNAs, snoRNAs, 7SK and, at lower extent, mRNAs(PubMed:25470060). In the nucleolus, localizes to rDNA locus, where it directly binds rRNAs and snoRNAs, and promotes rRNA transcription, processing and modification. Required for rRNA 2'-O-methylation, possibly by promoting the recruitment of late-acting snoRNAs SNORD56 and SNORD58 with pre-ribosomal complexes(PubMed:25470060, PubMed:25477391). In the nucleoplasm, binds 7SK RNA and is recruited to the promoters of Pol II-transcribed genes: acts by facilitating the release of P-TEFb from inhibitory 7SK snRNP in a manner that is dependent on its helicase activity, thereby promoting transcription of its target genes(PubMed:25470060). Functions as cofactor for JUN-activated transcription: required for phosphorylation of JUN at 'Ser-77'(PubMed:11823437, PubMed:25260534). Can unwind double-stranded RNA(helicase) and can fold or introduce a secondary structure to a single-stranded RNA(foldase)(PubMed:9461305). Involved in rRNA processing(PubMed:14559904, PubMed:18180292).

Molekulargewicht: 87 kDa

Gen-ID: 9188

UniProt: Q9NR30

Pathways: SARS-CoV-2 Protein Interaktom