Cullin 4A Antikörper (AA 460-759)

Produktnummer ABIN7113117

Dieser Anti-Cullin 4A Antikörper ist ein Maus Monoklonal Antikörper zur Detektion von Cullin 4A in WB, ELISA, IHC, IF und IP. Geeignet für Human, Maus, Ratte und Affe.

Kurzübersicht für Cullin 4A Antikörper (AA 460-759) (ABIN7113117)

Target: Cullin 4A (CUL4A)

Reaktivität: Human, Maus, Ratte, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cullin 4A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: 5A7

Produktdetails anti-Cullin 4A Antikörper

Bindungsspezifität: AA 460-759

Verwendungszweck: CUL4A antibody

Aufreinigung: Protein A+G purification

Reinheit: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 460-759aa

Immunogen: cullin 4A

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:2000, IP: 1:500-1:1000, IHC: 1:20-1:200, IF: 1:20-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze / thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C for 12 months

Haltbarkeit: 12 months

Details zu Cullin 4A

Target: Cullin 4A (CUL4A)

Andere Bezeichnung: CUL4A

Hintergrund:

Synonyms: Cullin-4A (CUL-4A)|CUL4A

Background: Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. DCX(DDB2) ubiquitinates histones H3-H4 and is required for efficient histone deposition during replication-coupled(H3.1) and replication-independent(H3.3) nucleosome assembly, probably by facilitating the transfer of H3 from ASF1A/ASF1B to other chaperones involved in histone deposition. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9. DCX(DTL) directs autoubiquitination of DTL.

Molekulargewicht: 88 kDa, 77 kDa

Gen-ID: 8451

UniProt: Q13619