CHCHD4 Antikörper

Produktnummer ABIN7112969

Der Kaninchen Polyklonal Anti-CHCHD4-Antikörper wurde für WB, ELISA, IHC, IF und IP validiert. Er ist geeignet, CHCHD4 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für CHCHD4 Antikörper (ABIN7112969)

Target: CHCHD4 (Coiled-Coil-Helix-Coiled-Coil-Helix Domain Containing 4 (CHCHD4))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CHCHD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Produktdetails anti-CHCHD4 Antikörper

Verwendungszweck: CHCHD4 antibody

Aufreinigung: Immunogen affinity purified

Reinheit: ≥95 % as determined by SDS-PAGE

Immunogen: coiled-coil-helix-coiled-coil-helix domain containing 4

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:2000, IP: 1:200-1:1000, IHC: 1:20-1:200, IF: 1:20-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze / thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C for 12 months

Haltbarkeit: 12 months

Details zu CHCHD4

Target: CHCHD4 (Coiled-Coil-Helix-Coiled-Coil-Helix Domain Containing 4 (CHCHD4))

Andere Bezeichnung: CHCHD4

Hintergrund:

Synonyms: Mitochondrial intermembrane space import and assembly protein 40|Coiled-coil-helix-coiled-coil-helix domain-containing protein 4|CHCHD4|MIA40

Background: Functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17 or MICU1(PubMed:16185709, PubMed:26387864, PubMed:19182799, PubMed:21059946, PubMed:23186364). Required for the import and folding of small cysteine-containing proteins(small Tim) in the mitochondrial intermembrane space(IMS)(PubMed:16185709, PubMed:19182799, PubMed:21059946). Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria(PubMed:16185709, PubMed:19182799, PubMed:21059946). The oxidized form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS(PubMed:16185709, PubMed:19182799, PubMed:21059946). Reduced CHCHD4/MIA40 is then reoxidized by GFER/ERV1 via a disulfide relay system(PubMed:23186364). Mediates formation of disulfide bond in MICU1 in the IMS, promoting formation of the MICU1-MICU2 heterodimer that regulates mitochondrial calcium uptake(PubMed:26387864).

Molekulargewicht: 22-25 kDa

Gen-ID: 131474

UniProt: Q8N4Q1