CHFR Antikörper (AA 41-140)

Produktnummer ABIN706211

Der Kaninchen Polyklonal Anti-CHFR-Antikörper wurde für ELISA, IHC (p), IF (cc), IF (p) und IHC (fro) validiert. Er ist geeignet, CHFR in Proben von Human zu detektieren.

Kurzübersicht für CHFR Antikörper (AA 41-140) (ABIN706211)

Target: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CHFR Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-CHFR Antikörper

Bindungsspezifität: AA 41-140

Homologie: Human,Mouse,Rat,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CHFR

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu CHFR

Target: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Andere Bezeichnung: CHFR

Hintergrund:

Synonyms: Checkpoint with forkhead and ring finger domains, Checkpoint with forkhead and RING finger domains protein, E3 ubiquitin protein ligase CHFR, EC 6.3.2., RING finger protein 196, RNF 116, RNF 196, RNF116, RNF196, Ubiquitin ligase protein, CHFR_HUMAN.

Background: A number of human cancers are sensitive to mitotic stress, implying checkpoint defects. Many proteins that contain forkhead-associated (FHA) domains are cell cycle checkpoints and CHFR is one such protein. It has a FHA and ring finger domain within its N terminus. Within its C terminus, CHFR contains a cysteine-rich region that did not display significant similarity to any protein in the GenBank database, but is highly conserved between humans and mice. It has been concluded that CHFR defines a checkpoint that delays entry into metaphase in response to mitotic stress. It has been found CpG methylation-dependent silencing of CHFR expression occurs in 45 % of cancer cell lines, 40 % of primary colorectal cancers, 53 % of colorectal adenomas, and 30 % of primary head and neck cancers.

Gen-ID: 55743