Rekombinanter EpCAM Antikörper

Produktnummer ABIN7072595

Produktdetails anti-EpCAM Antikörper

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EpCAM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Radioimmunoassay (RIA)

Verwendungszweck: Anti-EpCAM [HEA125], Mouse IgG1, kappa

Spezifität: This antibody is specific for the extracytoplasmic part of human EpCAM, a stable and widely-distributed marker expressed on the surface of many adenocarcinomas. It reacts with carcinomas derived from the colorectum, stomach, pancreas, liver, lung, mammary gland, ovary, thyroid, kidney, urinary bladder and prostate.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: This antibody was raised by immunising BALB/c mice with the human colon carcinoma cell line HT-29.

Klon: HEA125

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: This antibody has applications in the detection of normal and neoplastic epithelial cells, and distinguishing carcinomas from non-epithelial neoplasms (Momburg et al, 1987). This antibody has been used in radioimmunoassays on live cells and immunoenzymatic staining assays on glutaraldehyde-fixed cells, as well as in immunoperoxidase staining of frozen and formalin-fixed paraffin tissue sections (Moldenhauer et al, 1987, Momburg et al, 1987). These revealed it to react with most normal human epithelial cells, but not non-epithelial tissue, and to intensely react with human carcinomas and metastatic lesions, but not lymphoma, melanoma, sarcoma or neuroblastoma cell lines (Moldenhauer et al, 1987, Momburg et al, 1987). Squamous cell carcinomas stain less strongly than adenocarcinomas, and keratinizing areas of tumor masses remain negative (Momburg et al, 1987). This antibody has also been used in immunoprecipitation analysis to characterise human EpCAM (Moldenhauer et al, 1987), and in immunohistochemical analysis of EpCAM expression in formalin-fixed, paraffin-embedded liver tissue sections (Igarashi et al, 2013, Wang et al, 2018) and frozen liver tissue sections (Crosby et al, 1998). Additionally, it has been used in the immunocytochemical detection of EpCAM expression in mononuclear cell preparations obtained from human peripheral blood or lamina propria (Qiao et al, 1996).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Andere Bezeichnung: EpCAM (EPCAM Produkte)

Hintergrund: CD326, Ep-CAM, Epithelial cell adhesion molecule, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, EGP, Epithelial glycoprotein 314, EGP314, hEGP314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, HEA 125

UniProt: P16422