Rekombinanter gamma-Aminobutyric Acid Receptor Subunit beta (RDL) Antikörper

Produktnummer ABIN7072589

Der Kaninchen Chimeric Anti--Antikörper wurde für Crys und SEC-HPLC validiert. Er ist geeignet, in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter gamma-Aminobutyric Acid Receptor Subunit beta (RDL) Antikörper (ABIN7072589)

Target: gamma-Aminobutyric Acid Receptor Subunit beta (RDL)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Unkonjugiert

Applikation: Crystallization (Crys), Size-exclusion chromatography-High Pressure Liquid Chromatography (SEC-HPLC)

Klon: 1F4

Produktdetails

Verwendungszweck: Anti-GABAR [1F4], Rabbit IgG, kappa

Spezifität: This antibody recognises α1β2γ2 GABA-A receptor which is found in up to 50 % of the synapses in the brain. It's ligand, GABA is a major inhibitory transmitter in the nervous system, binds to this receptor and opens a channel permeable to chloride. Disfunctions of GABAR are found in anxiety, neurodevelopmental disorderds and epilepsy.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Aufreinigung: Protein A affinity purified

Immunogen: This antibody was raised against alpha1beta2gamma2 GABA-A receptor.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: FSEC: High affinity and specificity of the 1F4 antibody to α1β2γ2 GABA-A receptor was demonstrated using fluorescence-detection size exclusion chromatography with GFP-tagged receptor (shift in elution volume) (Zhu et al., 2018). Crystallography: Furthermore, this antibody was utilised in production and purification of the α1β2γ2 GABA-A receptor (Zhu et al., 2018). Finally, Fab fragments derived from the antibody were used to facilitate crystallisation and improve stabilisation of the α1β2γ2 GABA-A receptor (Zhu et al., 2018).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Antigendetails

Target: gamma-Aminobutyric Acid Receptor Subunit beta (RDL)

Andere Bezeichnung: GABAR

Hintergrund: Gamma-aminobutyric acid receptor A, Gamma-aminobutyric acid receptor, Gamma-aminobutyric acid receptor subunit alpha-1, Gamma-aminobutyric acid receptor subunit beta-2, Gamma-aminobutyric acid receptor subunit gamma-2, GABA(A), GABA-A receptor, α1β2γ2 GABA-A receptor

UniProt: P14867, P47870, P18507