Rekombinanter Fc epsilon RI/FCER1A Antikörper

Produktnummer ABIN7072531

Dieses Hamster Monoklonal-Antikörper erkennt spezifisch Fc epsilon RI/FCER1A in FACS, IHC, IP und Dep. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für Rekombinanter Fc epsilon RI/FCER1A Antikörper (ABIN7072531)

Target: Fc epsilon RI/FCER1A (FCER1A) (Fc Fragment of IgE Receptor Ia (FCER1A))

Antikörpertyp: Recombinant Antibody

Reaktivität: Maus

Wirt: Hamster

Klonalität: Monoklonal

Konjugat: Dieser Fc epsilon RI/FCER1A Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Depletion (Dep)

Klon: Mar-1

Produktdetails anti-Fc epsilon RI/FCER1A Antikörper

Verwendungszweck: Anti-FceR1 alpha [Mar-1], Hamster (Armenian) IgG, lambda

Spezifität: This antibody specifically targets the non-signalling alpha chain of the FceR1 complex.

Produktmerkmale:

Original Species of Ab: Hamster, Armenian

Original Format of Ab: IgG

Aufreinigung: Protein A affinity purified

Isotyp: IgG lambda

Anwendungsinformationen

Applikationshinweise: This antibody has been used in flow cytometric analysis of FceR1 alpha cell surface expression by basophils (Min et al, 2004, Chen et al, 2009), and immunohistochemical analysis of epidermal sheets (Holzmann et al, 2004). It has also been used in immunoprecipitation analysis of cell lysates from lung cDCs and MC/9 mast cells (Grayson et al, 2007). In vitro incubation of basophils with this antibody blocks subsequent MAR-1 staining, but does not activate basophil IL-4 production (Sokol et al, 2008). Intravenous treatment of mice with this antibody results in basophil depletion in peripheral blood, spleen, bone marrow and liver, but not skin or intraperitoneal mast cell depletion (Sokol et al, 2008). Following treatment with this antibody, basophil-depleted mice display impaired Th2 differentiation upon papain immunisation (Sokol et al, 2008). However, MAR-1 treated mice respond normally to high-dose intravenous Ig in both the K/BxN serum transfer arthritis and collagen-induced arthritis models, despite basophil depletion (Campbell et al, 2014).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu Fc epsilon RI/FCER1A

Target: Fc epsilon RI/FCER1A (FCER1A) (Fc Fragment of IgE Receptor Ia (FCER1A))

Andere Bezeichnung: FceR1 alpha

Hintergrund: Fcer1a, Fc epsilon RI alpha, FceRIa, FceRI alpha, FceRI-α, high affinity IgE receptor, High affinity immunoglobulin epsilon receptor subunit alpha,Fce1a, Fc-epsilon RI-alpha, FcERI, IgE Fc receptor subunit alpha

UniProt: P20489

Pathways: Fc-epsilon Rezeptor Signalübertragung, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process