Rekombinanter CD4 Antikörper

Produktnummer ABIN7072528

Dieses Kaninchen Chimeric-Antikörper erkennt spezifisch CD4 in FACS, ELISA und Neut. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rekombinanter CD4 Antikörper (ABIN7072528)

Target: CD4

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser CD4 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), ELISA, Neutralization (Neut)

Klon: DB81

Produktdetails anti-CD4 Antikörper

Verwendungszweck: Anti-CD4 [DB81], Rabbit IgG, kappa

Spezifität: This antibody is specific for human CD4, and recognises an epitope in the vicinity of a 13-amino acid loop in domain 2 of CD4. It cross-reacts with chimpanzee CD4, but not baboon or macaque CD4.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: This antibody was raised by immunising BALB/c mice with human CD4 complexed with HIV-1 Env (gp120).

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: This antibody preferentially binds to a complex-enhanced epitope on the D2 domain of human CD4. Plasmon resonance analysis has shown that the binding affinity of this antibody to the CD4-gp120 complex is almost one order of magnitude higher than its binding to CD4 alone (Burastero et al, 2011), while ELISA analysis has confirmed that the epitope recognised by this antibody does not involve gp120 (Burastero et al, 2011). This antibody, through a post-binding mechanism, inhibits the cell fusion and replication of a range of HIV-1 strains (Burastero et al, 2011). It does not exert inhibitory effects on T-cell activation in vitro, a useful property for clinical use.

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu CD4

Target: CD4

Andere Bezeichnung: CD4

Hintergrund: Lu/T4, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3

UniProt: P01730

Pathways: T-Zell Rezeptor Signalweg, Maintenance of Protein Location, CXCR4-mediated Signaling Events