Rekombinanter TIMD2 Antikörper (Extracellular Domain)

Produktnummer ABIN7072511

Der Kaninchen Chimeric anti-TIMD2 Antikörper wird verwendet zum Nachweis von TIMD2 in Proben von Maus. Er wurde validiert für FACS, WB, BP, IHC und Func.

Kurzübersicht für Rekombinanter TIMD2 Antikörper (Extracellular Domain) (ABIN7072511)

Target: TIMD2 (T Cell Immunoglobulin and Mucin Domain Containing 2 (TIMD2))

Antikörpertyp: Recombinant Antibody

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser TIMD2 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Blocking Peptide (BP), Immunohistochemistry (IHC), Functional Studies (Func)

Klon: RMT2-14

Produktdetails anti-TIMD2 Antikörper

Bindungsspezifität: AA 1-230, Extracellular Domain

Verwendungszweck: Anti-TIM-2 [RMT2-14], Rabbit IgG, lambda

Spezifität: This antibody is specific for murine TIM-2, a transmembrane protein that is expressed on activated T cells and preferentially on differentiated Th2 cells. A blockade of TIM-2/TIM-2 ligand interaction results in T cell hyperproliferation and Th2 cytokine production. TIM-2 plays a critical role in the development of atopic disease and other Th2-biased immune responses.

Produktmerkmale:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Aufreinigung: Protein A affinity purified

Immunogen: This antibody was raised by immunising Sprague Dawley rats with TIM-2-Ig consisting of the extracellular domain (aa 1-230 of mouse TIM-2) and the Fc portion of mouse IgG2a, emulsified in complete Freund's adjuvant and then fusing lymph node cells from the immunized rats with P3U1 myeloma cells.

Isotyp: IgG lambda

Anwendungsinformationen

Applikationshinweise: This antibody has been used in various FACS analyses, for instance, to evaluate the expression of TIM family members on mouse mast cells (Nakae et al, 2007) and to block the H-ferritin binding to TIM-2/L5178Y cells (Kawamoto et al, 2011). This antibody has been used in FACS and immunohistochemistry analyses to show that TIM-2 is expressed on mouse fetal hepatocytes (Watanabe et al, 2007). This antibody has also been used in Western Blots and for blocking experiments in ferritin binding analysis, which suggests that Tim-2 is the H-ferritin receptor on oligodendrocytes (Todorich et al, 2008). In addition, this antibody has been used in collagen-induced arthritis (CIA) models to determine whether TIM-2 contributes to the development of T helper (Th) 1 or Th17 cells and joint inflammation (Kawamoto et al, 2011).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu TIMD2

Target: TIMD2 (T Cell Immunoglobulin and Mucin Domain Containing 2 (TIMD2))

Andere Bezeichnung: TIM-2

Hintergrund: TIMD2, T Cell Immunoglobulin Mucin-2, T Cell immunoglobulin and mucin domain protein 2, TIM3

UniProt: Q8R183