Rekombinanter Hemoglobin Antikörper

Produktnummer ABIN7072452

Dieses Anti-Hemoglobin-Antikörper ist ein Kaninchen Chimeric-Antikörper zur Detektion von Hemoglobin in WB und ELISA. Geeignet für Maus.

Kurzübersicht für Rekombinanter Hemoglobin Antikörper (ABIN7072452)

Target: Hemoglobin

Antikörpertyp: Recombinant Antibody

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser Hemoglobin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Klon: 2A1

Produktdetails anti-Hemoglobin Antikörper

Verwendungszweck: Anti-Haemoglobin [2A1], Rabbit IgG, kappa

Spezifität: 2A1 exhibited polyreactive recognition of peptide 100-119 from the alpha subunit and peptide 100-119 frmo the beta subunit. Moreover, 2A1 bound Hb and the Hb-haptoglobin complex with equal efficiency, implying different binding sites on Hb for haptoglobin and 2A1. 2A1 was also found to distinguish between Hb and metHb.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG

Aufreinigung: Protein A affinity purified

Immunogen: Murine hybridomas were generated using spleen cells from aging (8-10 month old) NZB/W F1 animals. Hybridomas secreting antibodies reactive to murine hemoglobin were subcloned by limiting dilution and antibody isotypes determined using commercial reagents. Eight-week-old NZB/W F1 were immunized subcutaneously with 50 mg mHb antigen emulsified in Complete Fruend's Adjuvant.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: 2A1 has been used to bind to mHb in Western Blots and has been found to increase the secretion of TNF-alpha from endothelial cells when co-incubated with Hb, suggesting that 2A1 induces pro-inflammatory events (Bhatnagar 2011).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu Hemoglobin

Target: Hemoglobin

Andere Bezeichnung: Haemoglobin

Hintergrund: Hemoglobin, Hb

UniProt: P01942