Rekombinanter CD16 Antikörper

Produktnummer ABIN7072393

Dieses Kaninchen Chimeric-Antikörper erkennt spezifisch CD16 in FACS, IP, IHC, BR, Dep und SPR. Er zeigt eine Reaktivität gegenüber Human, Rhesusaffen, Cynomolgus, Pavian, Capuchin Monkey, Schimpanse, Macaca nemestrina, Marmoset, Saguinus oedipus, Sooty mangabey (Cercocebus atys) und Squirrel Monkey.

Kurzübersicht für Rekombinanter CD16 Antikörper (ABIN7072393)

Target: CD16

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Rhesusaffen, Cynomolgus, Pavian, Capuchin Monkey, Schimpanse, Macaca nemestrina, Marmoset, Saguinus oedipus, Sooty mangabey (Cercocebus atys), Squirrel Monkey

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser CD16 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Blocking Reagent (BR), Depletion (Dep), Surface Plasmon Resonance (SPR)

Klon: 3G8

Produktdetails anti-CD16 Antikörper

Verwendungszweck: Anti-Fc gamma receptor III [3G8], Rabbit IgG, kappa

Spezifität: 3G8 binds to CD16 at an epitope which is not the IgG-binding site. This antibody cannot distinguish between CD16a transmembrane isoform found on the surface of NK cells and macrophages (which can trigger the cytolytic response), and the GPI-linked CD16b isoform present on neutrophils or soluble CD16 found in the plasma. CD16 is the low affinity IgG receptor III and binds to aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis and ADCC (antibody-dependent cell-mediated cytotoxicity).

Kreuzreaktivität: Pavian, Capuchin Monkey, Schimpanse, Cynomolgus, Macaca nemestrina, Marmoset, Rhesusaffen, Saguinus oedipus, Sooty mangabey (Cercocebus atys), Squirrel Monkey

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: 3G8 was prepared by immunizing mice with human PMN (polymorphonuclear leukocytes) cells.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: 3G8 antibody was used to construct a recombinant bispecific scFv (bsscFv) along with the mAb clone F3.3 anti-HLA class II antibody (connected by a (Gly4Ser)4 linker)which was expressed in SF21 insect cells. This bsscFv allows NK cells to target malignant B lymphoid cells and mediates effector cell lysis (ASCC) of the malignant cells (also lysis of primary leukaemia samples). 3G8 is also able to block neutrophil phagocytosis and stimulates NK cell proliferation, and can be used in FC, SPR, IP and IHC studies.

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu CD16

Target: CD16

Andere Bezeichnung: Fc gamma receptor III

Hintergrund: CD16, low affinity IgG receptor III, FcγRIII, low affinity FcγRIII

UniProt: Q9ULV2

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process