Rekombinanter MUC1 Antikörper

Produktnummer ABIN7072292

Der Kaninchen Chimeric Anti-MUC1-Antikörper wurde für IHC, WB, ELISA, IP und IRMA validiert. Er ist geeignet, MUC1 in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter MUC1 Antikörper (ABIN7072292)

Target: MUC1 (Mucin 1 (MUC1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser MUC1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunoradiometric Assay (IRMA)

Klon: Mc5

Produktdetails anti-MUC1 Antikörper

Verwendungszweck: Anti-MUC1 [Mc5], Rabbit IgG, kappa

Spezifität: This antibody is specific for the amino acid sequence DTRPAP of MUC1. The alpha subunit of MUC1 has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit of MUC-1 contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG2

Aufreinigung: Protein A affinity purified

Immunogen: Human breast epithelial mucin.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: In an attempt to develop a reliable way to detect breast cancer, immunoprecipitation was preformed on NPGP from HMFG using the mouse version of this antibody bound to Sepharose 4B. Furthermore, this antibody was tested in an radioimunnology experiment on NPGP using the mouse version of this antibody (Ceriani et al, 1992, pmid:1377884). The epitope of this antibody was mapped by ELISA using small peptides - the murine version of this antibody was used (Petrakou et al, 1998 , pmid:9422085). This antibody was used to identify cells expressing the MUC1 protein. This was accomplished by using the mouse version of this antibody on cell lysates of cell transfected with the MUC1 protein in a western blot analysis (Rubinstein et al, 2006, pmid:17145869). To compare the diagnostic usefulness of different antibodies, the mouse version of this antibody was used for immunohistochemistry on cell pellets from pleural fluid specimens (Creaney et al, 2008, pmid:18454162). While developing an antibody against the alpha/beta junction of MUC1 the mouse version of this antibody was used for western blot on the MUC1 protein (Rubinstein et al, 2008, pmid:18821582). While assesing a panel of 56 anti MUC1 antibodies for their ability to detect cancer the mouse version of this antibody was used for an immunoradiometric assay on a serum made from sera from 10 different patients with breast, ovarian or colon cancer (Norum et al, 1998, pmid:9422099). To more accurately determine the epitope of this antibody, an ELISA was preformed using the murine version of this antibody on different small pepetides from the MUC1 protein (Karsten et al, 2004, pmid:15115750).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2 format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu MUC1

Target: MUC1 (Mucin 1 (MUC1))

Andere Bezeichnung: MUC1

Hintergrund: CD227, BEM, Breast epithelial mucin, Mucin-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, CA 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, KL-6, PEMT, Peanut-reactive urinary mucin, PUM, Polymorphic epithelial mucin, PEM, Tumor-associated epithelial membrane antigen, EMA, Tumor-associated mucin

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling