Rekombinanter Fc gamma RII (CD32) Antikörper

Produktnummer ABIN7072239

Dieses Anti--Antikörper ist ein Kaninchen Chimeric-Antikörper zur Detektion von in FACS, WB, IHC, IF, St und BP. Geeignet für Maus.

Kurzübersicht für Rekombinanter Fc gamma RII (CD32) Antikörper (ABIN7072239)

Target: Fc gamma RII (CD32)

Antikörpertyp: Recombinant Antibody

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Stimulation (St), Blocking Peptide (BP)

Klon: AT130-5

Produktdetails

Verwendungszweck: Anti-CD32 [AT130-5], Rabbit IgG, kappa

Spezifität: This antibody is specific for murine CD32, an Fc gamma surface receptor protein with a low affinity for IgG antibodies that expresses on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. CD32 binds antibody-antigen immune complexes and mediates adaptive immune responses.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c FcgammaRIIB^-/- mice with FcgammaRIIB-rat CD4 domain 3/4 fusion protein.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: The original version of this antibody (mouse IgG1) was used in various FACS, Western blot, and immunofluorescence experiments to demonstrate that, for example, interaction with FcγRIIB is critical for the agonistic activity of anti-CD40 mAb (White et al., 2011, PMID: 21742972), and that immunotherapy targeting murine FcγRIIB is limited by mAb consumption and receptor internalization (Williams et al., 2013, PMID: 24026082). It is noted that, unlike the antagonistic anti-CD32 (clone AT128) antibody, which blocks FcγRIIB receptor phosphorylation, this agonistic anti-CD32 (clone AT130-5) has been shown to activate receptor function while blocking immune complex engagement to the inhibitory FcγRs (Vollack et al., 2017, PMID: 28492697). These differential antagonistic/agonistic anti-CD32 mAbs have been used to probe more specifically the role of CD32 in the murine FVIII-specific recall response (Vollack et al., 2017, PMID: 28492697).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Antigendetails

Target: Fc gamma RII (CD32)

Andere Bezeichnung: CD32

Hintergrund: FcγRIIB, FcgRIIb, CD32B, CDw32, FCG2, FCGR2, FCGR2B, IGFR2, Immunoglobulin G Fc receptor II, Low affinity immunoglobulin gamma Fc region receptor II-b, MGC23887, MGC30032, RP11-5K23.6

UniProt: P08101