Rekombinanter CD8 alpha/lyt-2 Antikörper

Produktnummer ABIN7072217

Der Ratte Chimeric anti-CD8 alpha/lyt-2 Antikörper wird verwendet zum Nachweis von CD8 alpha/lyt-2 in Proben von Maus. Er wurde validiert für Dep, FACS, IHC, IHC (fro) und in vivo.

Kurzübersicht für Rekombinanter CD8 alpha/lyt-2 Antikörper (ABIN7072217)

Target: CD8 alpha/lyt-2

Antikörpertyp: Recombinant Antibody

Reaktivität: Maus

Wirt: Ratte

Klonalität: Chimeric

Konjugat: Unkonjugiert

Applikation: Depletion (Dep), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), In vivo Studies (in vivo)

Klon: YTS 169-4

Produktdetails

Verwendungszweck: Anti-CD8 alpha/Lyt-2 [YTS 169.4], Rat IgG2b, kappa

Spezifität: This antibody recognises the murine CD8 cell surface antigen expressed by a subset of T lymphocytes.

Produktmerkmale:

Original Species of Ab: Rat

Original Format of Ab: IgG2b

Aufreinigung: Protein A affinity purified

Immunogen: Murine CD8 (Lyt-2).

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: The antibody has been shown to show depleting activity in vivo. Mice have two alleles for CD8, Lyt2.1, and Lyt2.2, which are restricted to certain mouse strains. Lyt2.1, for example, is expressed in the mouse strains CBA, AKR, C3H, and DBA, whereas Lyt2.2 is expressed in the mouse strains BALB/c and C57BL/6 (B/6). YTS 169.4 was originally raised against CBA mouse thymocytes. However, there are publications in which it has been used in C57BL/6 and BALB/c mice, wherein it was used specifically as a pan-reactive Lyt2 reagent (Tavaré et al., 2014, PMID: 24390540).

Kommentare:

This is a chimeric antibody created to reduce immunogenicity during in vivo applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Antigendetails

Target: CD8 alpha/lyt-2

Hintergrund: Leu2, CD8, CD8A, CD8a molecule, CD8a antigen, Leu2 T-lymphocyte antigen, Lyt-2, Lyt2OKT8, p32T-cell antigen Leu2, T-cell antigen, T cell co-receptor, T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2, T8 T-cell antigen

UniProt: P01731