TMEM119 Antikörper (Extracellular, N-Term)

Produktnummer ABIN7886684

Der Kaninchen Polyklonal anti-TMEM119 Antikörper (ABIN7886684) detektiert spezifisch TMEM119 in FACS und WB. Dieser Antikörper reagiert spezifisch mit Proben aus Maus.

Kurzübersicht für TMEM119 Antikörper (Extracellular, N-Term) (ABIN7886684)

Target: TMEM119 (Transmembrane Protein 119 (TMEM119))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TMEM119 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB)

Produktdetails anti-TMEM119 Antikörper

Bindungsspezifität: AA 53-67, Extracellular, N-Term

Verwendungszweck: A Rabbit Polyclonal Antibody to TMEM119 (extracellular)

Kreuzreaktivität: Maus, Ratte

Kreuzreaktivität (Details): This antibody will not recognize human TMEM119.

Homologie: Rat - 12,15 amino acid residues identical

Produktmerkmale: Anti-TMEM119 (extracellular) Antibody (ABIN7043859, ABIN7044740 and ABIN7044741) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry. It has been designed to recognize TMEM119 from rat and mouse samples. The antibody will not recognize the protein from human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)GTPAFSPTPERPQPT, corresponding to amino acid residues 53 - 67 of mouse TMEM119 

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7237517)

Blocking Peptide: (ABIN7237517)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu TMEM119

Target: TMEM119 (Transmembrane Protein 119 (TMEM119))

Andere Bezeichnung: TMEM119

Hintergrund:

Synonyms: Transmembrane Protein 119, Osteoblast induction factor, OBIF

Description: Transmembrane protein 119, Tmem119, is a bone formation related factor that belongs to a group of bone morphogenetic proteins (BMPs). It is a class IA single pass transmembrane protein1.Tmem119 promotes the differentiation of myoblasts (embryonic precursors of muscle cells) into osteoblasts and plays a role in numerous BMP signaling pathways1,2.Tmem119 is uniquely expressed in microglia cells and it is therefore used as a microglia-specific homeostatic marker that can assist in differentiating microglia cells from peripherally derived macrophages in the brain3. This marker is very important for diagnosing CNS disease: monocytes/macrophages are key cellular modulators of brain homeostasis. In the healthy brain resident microglia are the predominant macrophage cell population but when the blood-brain barrier is leaking peripheral monocytes/macrophages can infiltrate the brain and participate in CNS disease pathogenesis. Distinguishing these two monocytesmacrophages populations is often challenging and hence the existence of a highly expressed and robust microglia marker such as Tmem119 is very important4.

Gen-ID: 231633

NCBI Accession: NM_181724

UniProt: Q8R138