TMEM119 Antikörper (Extracellular)

Produktnummer ABIN7043859

Produktdetails anti-TMEM119 Antikörper

Target: TMEM119 (Transmembrane Protein 119 (TMEM119))

Bindungsspezifität: AA 53-67, Extracellular

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TMEM119 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc))

Verwendungszweck: A Rabbit Polyclonal Antibody to TMEM119 (extracellular)

Spezifität: Extracellular, N-terminus.

Kreuzreaktivität: Maus, Ratte

Kreuzreaktivität (Details): This antibody will not recognize human TMEM119.

Homologie: Rat - 12,15 amino acid residues identical

Produktmerkmale: Anti-TMEM119 (extracellular) Antibody (ABIN7043859, ABIN7044740 and ABIN7044741) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry. It has been designed to recognize TMEM119 from rat and mouse samples. The antibody will not recognize the protein from human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)GTPAFSPTPERPQPT, corresponding to amino acid residues 53 - 67 of mouse TMEM119

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: (ABIN7237517)

Blocking Peptide: (ABIN7237517)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu TMEM119

Target: TMEM119 (Transmembrane Protein 119 (TMEM119))

Andere Bezeichnung: TMEM119 (TMEM119 Produkte)

Hintergrund: Transmembrane Protein 119, Osteoblast induction factor, OBIF,Transmembrane protein 119, Tmem119, is a bone formation related factor that belongs to a group of bone morphogenetic proteins (BMPs). It is a class IA single pass transmembrane protein1.Tmem119 promotes the differentiation of myoblasts (embryonic precursors of muscle cells) into osteoblasts and plays a role in numerous BMP signaling pathways1,2.Tmem119 is uniquely expressed in microglia cells and it is therefore used as a microglia-specific homeostatic marker that can assist in differentiating microglia cells from peripherally derived macrophages in the brain3. This marker is very important for diagnosing CNS disease: monocytes/macrophages are key cellular modulators of brain homeostasis. In the healthy brain resident microglia are the predominant macrophage cell population but when the blood-brain barrier is leaking peripheral monocytes/macrophages can infiltrate the brain and participate in CNS disease pathogenesis. Distinguishing these two monocytesmacrophages populations is often challenging and hence the existence of a highly expressed and robust microglia marker such as Tmem119 is very important4.

Alternative names: Transmembrane Protein 119, Osteoblast induction factor, OBIF

Gen-ID: 231633

NCBI Accession: NM_181724

UniProt: Q8R138