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TMEM119 Antikörper (Extracellular)

Dieses Anti-TMEM119-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von TMEM119 in FACS und WB. Geeignet für Maus.
Produktnummer ABIN7043859

Kurzübersicht für TMEM119 Antikörper (Extracellular) (ABIN7043859)

Target

TMEM119 (Transmembrane Protein 119 (TMEM119))

Reaktivität

  • 4
  • 2
  • 1
Maus

Wirt

  • 5
  • 3
  • 1
Kaninchen

Klonalität

  • 4
  • 3
  • 1
Polyklonal

Konjugat

  • 6
  • 1
  • 1
Dieser TMEM119 Antikörper ist unkonjugiert

Applikation

  • 6
  • 5
  • 3
  • 3
  • 1
  • 1
Flow Cytometry (FACS), Western Blotting (WB)
  • Bindungsspezifität

    • 2
    • 2
    • 1
    • 1
    AA 53-67, Extracellular

    Verwendungszweck

    A Rabbit Polyclonal Antibody to TMEM119 (extracellular)

    Spezifität

    Extracellular, N-terminus.

    Kreuzreaktivität

    Maus, Ratte

    Kreuzreaktivität (Details)

    This antibody will not recognize human TMEM119.

    Homologie

    Rat - 12,15 amino acid residues identical

    Produktmerkmale

    Anti-TMEM119 (extracellular) Antibody (ABIN7043859, ABIN7044740 and ABIN7044741) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry. It has been designed to recognize TMEM119 from rat and mouse samples. The antibody will not recognize the protein from human samples.

    Aufreinigung

    Affinity purified on immobilized antigen.

    Immunogen

    Immunogen: Synthetic peptide

    Immunogen Sequence: (C)GTPAFSPTPERPQPT, corresponding to amino acid residues 53 - 67 of mouse TMEM119

    Isotyp

    IgG
  • Applikationshinweise

    Antigen preadsorption control: 1 μg peptide per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

    Application Dilutions Western blot wb: 1:200

    Kommentare

    Negative Control: (ABIN7237517)

    Blocking Peptide: (ABIN7237517)

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

    Konzentration

    1 mg/mL

    Buffer

    PBS pH 7.4

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • Target

    TMEM119 (Transmembrane Protein 119 (TMEM119))

    Andere Bezeichnung

    TMEM119

    Hintergrund

    Transmembrane Protein 119, Osteoblast induction factor, OBIF,Transmembrane protein 119, Tmem119, is a bone formation related factor that belongs to a group of bone morphogenetic proteins (BMPs). It is a class IA single pass transmembrane protein1.Tmem119 promotes the differentiation of myoblasts (embryonic precursors of muscle cells) into osteoblasts and plays a role in numerous BMP signaling pathways1,2.Tmem119 is uniquely expressed in microglia cells and it is therefore used as a microglia-specific homeostatic marker that can assist in differentiating microglia cells from peripherally derived macrophages in the brain3. This marker is very important for diagnosing CNS disease: monocytes/macrophages are key cellular modulators of brain homeostasis. In the healthy brain resident microglia are the predominant macrophage cell population but when the blood-brain barrier is leaking peripheral monocytes/macrophages can infiltrate the brain and participate in CNS disease pathogenesis. Distinguishing these two monocytesmacrophages populations is often challenging and hence the existence of a highly expressed and robust microglia marker such as Tmem119 is very important4.

    Alternative names: Transmembrane Protein 119, Osteoblast induction factor, OBIF

    Gen-ID

    231633

    NCBI Accession

    NM_181724

    UniProt

    Q8R138
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