SNCA Antikörper (Intracellular)

Produktnummer ABIN7043800

Dieses Meerschweinchen Polyklonal-Antikörper erkennt spezifisch SNCA in WB und IHC. Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für SNCA Antikörper (Intracellular) (ABIN7043800)

Target: SNCA (Synuclein, alpha (SNCA))

Reaktivität: Ratte

Wirt: Meerschweinchen

Klonalität: Polyklonal

Konjugat: Dieser SNCA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-SNCA Antikörper

Bindungsspezifität: AA 97-110, Intracellular

Verwendungszweck: A Guinea Pig Polyclonal Antibody to α-Synuclein

Spezifität: Intracellular, C-terminus

Kreuzreaktivität: Maus, Ratte

Homologie: Mouse - identical, human - 11 out of 14 amino acid residues identical

Produktmerkmale: Guinea Pig Anti-Alpha-Synuclein Antibody is a highly specific antibody directed against an epitope of rat α-Synuclein (SNCA). The antibody can be used in western blot analysis and immunohistochemistry applications. It has been designed to recognize α-Synuclein from rat, mouse and human samples. The antigen used to immunize guinea pigs is the same as Anti-Alpha-Synuclein Antibody (ABIN7043799, ABIN7045180 and ABIN7045181) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KDQMGKGEEGYPQE, corresponding to amino acid residues 97-110 of rat alpha-synuclein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:400

Kommentare:

Negative Control: (ABIN7237358)

Blocking Peptide: (ABIN7237358)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu SNCA

Target: SNCA (Synuclein, alpha (SNCA))

Andere Bezeichnung: SNCA

Hintergrund: α-Synuclein, Non-A4 component of amyloid precursor, Parkinson disease (autosomal dominant, Lewy body) 4, PARK1, PARK4, NACP, SNCA,α-Synuclein belongs to the Synuclein family of proteins containing three other members (β-synuclein, γ-synuclein and synoretnin). α-Synuclein is a highly conserved acidic protein that is concentrated mainly in presynaptic axon terminals.The gene encoding α-synuclein is known as SNCA or PARK1. α-Synuclein, together with other members of its family, lacks typical secondary structure. In its primary structure, α-synuclein is characterized by seven imperfect 11-residue repeat sequences that are predicted to form five amphipatic helices on the amino terminal half. The acidic, glutamate rich carboxy-terminal region remains unstructured even in the presence of membranes. α-Synuclein helices 1-4 are predicted to associate with lipid vesicles while helix 5 would be responsible for protein-to-protein interaction. The protein interacts and with DAT, the dopamine transporter, and thus is involved in dopamine signaling. Under pathological conditions, α-synuclein can undergo conformational changes leading to protein aggregation and deposition.α-Synuclein shares structural homology with the 14-3-3 family of chaperon proteins and its expression is upregulated during periods of accelerated neuronal activity and plasticity thus suggesting it might be functioning as a molecular chaperon capable of binding to other intercellular proteins.α-Synuclein has been implicated in the pathophysiology of Parkinson's disease (PD) and Lewy body dementia (DLB). Mutations A30P and A3T are associated with PD while mutation E46K has been linked to DLB. These mutated α-synucleins form fibrils more rapidly than the wild-type protein in aqueous solution1. α-Synucleins are the major components of Lewy bodies and Lewy neurites in both diseases2.

Alternative names: α-Synuclein, Non-A4 component of amyloid precursor, Parkinson disease (autosomal dominant, Lewy body) 4, PARK1, PARK4, NACP, SNCA

Gen-ID: 29219

NCBI Accession: NM_000345

UniProt: P37377

Pathways: Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity