SYN2 Antikörper (Domain G, Intracellular)

Produktnummer ABIN7886619

Der Kaninchen Polyklonal anti-SYN2 Antikörper (ABIN7886619) detektiert spezifisch SYN2 in WB, IHC und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Ratte.

Kurzübersicht für SYN2 Antikörper (Domain G, Intracellular) (ABIN7886619)

Target: SYN2 (Synapsin II (SYN2))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SYN2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-SYN2 Antikörper

Bindungsspezifität: AA 438-450, Domain G, Intracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to Synapsin II

Kreuzreaktivität: Maus, Ratte

Kreuzreaktivität (Details): This antibody will recognize both canonical isoforms (also known as Synapsin IIa) and the shorter isoform (Synapsin-IIb).

Homologie: Mouse - identical, human- 12,13 amino acid residues identical

Produktmerkmale: Anti-Synapsin II (SYN2) Antibody (ABIN7043787, ABIN7044660 and ABIN7044661) is a highly selective antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Synapsin II from mouse, human, and rat samples. This antibody will recognize both canonical isoforms (also known as Synapsin IIa) and the shorter isoform (Synapsin IIb).

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)SGTLKEPDSSKTP, corresponding to amino acid residues 438-450 of rat Synapsin-2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7237311)

Blocking Peptide: (ABIN7237311)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu SYN2

Target: SYN2 (Synapsin II (SYN2))

Andere Bezeichnung: SYN2

Hintergrund:

Synonyms: Synapsin-2

Description: Synapsins are a family of ten homologous neuronal phosphoproteins evolutionarily conserved in invertebrate and vertebrate organisms. Synapsins appear to be crucial determinants for the fine-tuning of synaptic transmission and for synaptic remodeling. This is particularly evident in several paradigms of short-term plasticity (STP), which are specifically affected by mutations or malfunctioning of synapsin proteins. Notably, knockout of synapsin-2 in mice causes fully generalized forebrain seizures from the age of 2 months1.All synapsins, including synapsin-2, are composed of a highly homologous N-terminal region, and a more variable C-terminus. The N-terminal homologous region can be divided in three domains, namely domain A, B and C, which, except for domain B, are highly conserved across isoforms and species. Domain A is contains the phosphorylation site for PKA and CaMKI. Domain B, rich in small amino acids, is considered as a linker region connecting domain A to domain C. Domain C is a large region of about 300 amino acids, containing both hydrophobic and highly charged sequences with the potential to assume α-helix and β-sheet conformations. It shows extensive homology amongst the various members of the family, and mediates the core functions of synapsins1.

Gen-ID: 29179

NCBI Accession: NM_133625

UniProt: Q63537