SLC3A1 Antikörper (Extracellular)

Produktnummer ABIN7043713

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SLC3A1 in WB. Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für SLC3A1 Antikörper (Extracellular) (ABIN7043713)

Target: SLC3A1 (Solute Carrier Family 3 Member 1 (SLC3A1))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SLC3A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-SLC3A1 Antikörper

Bindungsspezifität: AA 277-291, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to SLC3A1

Spezifität: Extracellular, C-terminus

Kreuzreaktivität: Maus, Ratte

Homologie: Mouse - identical, human - 14,15 amino acid residues identical

Produktmerkmale: Anti-SLC3A1 (extracellular) Antibody (ABIN7043713, ABIN7044829 and ABIN7044830) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and can potentially be used for detecting the protein in living cells. It has been designed to recognize SLC3A1 from rat, mouse, and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KEQPDLNFRNPAVQE, corresponding to amino acid residues 277-291 of rat SLC3A1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400

Kommentare:

Negative Control: (ABIN7237055)

Blocking Peptide: (ABIN7237055)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu SLC3A1

Target: SLC3A1 (Solute Carrier Family 3 Member 1 (SLC3A1))

Andere Bezeichnung: SLC3A1

Hintergrund: Neutral and basic amino acid transport protein rBAT, Solute carrier family 3 (cystine, dibasic and neutral amino acid transporters) member 1, B(0,+)-type amino acid transport protein, NBAT, Amino acid transporter 1, ATR1,The heteromeric amino acid transporter (HAT) family is one of the major amino acid transporter families responsible for cellular uptake and epithelial transport. The uptake of cysteine mainly depends on HATs. HATs are localized to the plasma membrane and form heterodimers composed of a 12-membrane spanning light chain (SLC7) and a single membrane spanning heavy chain, SLC3. SLC7 catalyzes transport functions while SLC3 is essential for plasma membrane localization and stabilization of the light chains. The SLC3A1 is mainly expressed in the apical plasma membrane in the epithelial cells of intestinal mucosa and the renal proximal tubules1,2. Two heavy chains, SLC3A1 and SLC3A2 are covalently bound to the light chains through a disulfide bridge. SLC3A1 has been known to form a heterodimer only with SLC7A9, a family member of light chain1,2.Type 1 Cystinuria is associated with mutations in SLC3A1, an inherited disease characterized by the formation of cystine calculi in the kidneys, ureters, and bladder3.

Alternative names: SLC3A1, Neutral and basic amino acid transport protein rBAT, Solute carrier family 3 (cystine, dibasic and neutral amino acid transporters) member 1, B(0,+)-type amino acid transport protein, NBAT, Amino acid transporter 1, ATR1

Gen-ID: 29484

NCBI Accession: NM_000341

UniProt: Q64319