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RYR1 Antikörper (Intracellular)

RYR1 Reaktivität: Human WB, IHC, IF, IC Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN7043604
  • Target Alle RYR1 Antikörper anzeigen
    RYR1 (Ryanodine Receptor 1 (Skeletal) (RYR1))
    Bindungsspezifität
    AA 1371-1386, Intracellular
    Reaktivität
    • 22
    • 10
    • 2
    • 1
    • 1
    • 1
    Human
    Wirt
    • 20
    • 2
    Kaninchen
    Klonalität
    • 21
    • 2
    Polyklonal
    Konjugat
    • 10
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser RYR1 Antikörper ist unkonjugiert
    Applikation
    • 11
    • 9
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)
    Verwendungszweck
    A Rabbit Polyclonal Antibody to Ryanodine Receptor 1
    Spezifität
    Intracellular, N-terminus
    Kreuzreaktivität
    Human, Maus, Ratte
    Homologie
    Rat,mouse - identical
    Produktmerkmale
    Anti-Ryanodine Receptor 1 Antibody (ABIN7043604, ABIN7045211 and ABIN7045212)) is a highly specific antibody directed against an epitope of human RyR1. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize RyR1 from human, rat, and mouse samples.
    Aufreinigung
    Affinity purified on immobilized antigen.
    Immunogen

    Immunogen: Synthetic peptide

    Immunogen Sequence: (C)RAENEKDATTEKNKKR, corresponding to amino acid residues 1371-1386 of human RyR1

    Isotyp
    IgG
  • Applikationshinweise

    Antigen preadsorption control: 1 μg peptide per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

    Application Dilutions Western blot wb: 1:200

    Kommentare

    Cited Application: IP|IHC|IFC

    Negative Control: BLP-RR001

    Blocking Peptide: BLP-RR001

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
    Konzentration
    1 mg/mL
    Buffer
    PBS pH 7.4
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • Target
    RYR1 (Ryanodine Receptor 1 (Skeletal) (RYR1))
    Andere Bezeichnung
    RYR1 (RYR1 Produkte)
    Hintergrund
    RyR1, Skeletal muscle-type ryanodine receptor,It is well established that cytosolic calcium (Ca2+) acts as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The primary intracellular Ca2+ storage/release organelle in most cells is the endoplasmic reticulum (ER) or the sarcoplasmic reticulum (SR) in striated muscle cells.The ER and SR contain two Ca2+ release channel families, the inositol trisphosphate receptors (IP3Rs) and the ryanodine receptors (RyRs).3The ryanodine receptor family consists of three different isoforms: the skeletal muscle isoform, ryanodine receptor type 1 (RyR1), the cardiac muscle isoform, ryanodine receptor type 2 (RyR2) and the brain isoform, ryanodine receptor type 3 (RyR3).3The ryanodine receptors are homotetrameric proteins, which upon assembly create a Ca2+ conducting channel. They play a key role in the mechanism of excitation-contraction coupling in striated muscle. Binding of ryanodine (a poisonous alkaloid found in the South American plant Ryania speciosa) to the ryanodine receptor causes two major changes in the channel: a reduction in single-channel conductance and a marked increase in the open state probability, leading to an overall increase/decrease in the Ca2+ release capability of the channel.RyR1 is expressed predominantly in skeletal muscle and in areas of the brain, human RyR1 has at least three known alternative splice variants.Several diseases are attributed to mutations in the RyR1 gene: Central core disease (CCD), multi-minicore disease (MmD) and Malignant hyperthermia (MH) 4.

    Alternative names: Ryanodine Receptor 1, RyR1, Skeletal muscle-type ryanodine receptor
    Gen-ID
    6261
    NCBI Accession
    NM_000540
    UniProt
    P21817
    Pathways
    Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development
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