RTN4R Antikörper (Extracellular)

Produktnummer ABIN7043601

Produktdetails anti-RTN4R Antikörper

Target: RTN4R (Reticulon 4 Receptor (RTN4R))

Bindungsspezifität: AA 366-381, Extracellular

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RTN4R Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Verwendungszweck: A Rabbit Polyclonal Antibody to Nogo Receptor

Spezifität: Extracellular

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse,rat - 15,16 amino acid residues identical

Produktmerkmale: Anti-Nogo Receptor (extracellular) Antibody is directed against an extracellular epitope of the human Nogo receptor. Anti-Nogo Receptor (extracellular) Antibody (ABIN7043601, ABIN7044757 and ABIN7044758) can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize NgR from human, mouse and rat samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)GDSPPGNGSGPRHIND corresponding to amino acid residues 366-381 of the human Nogo receptor

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Kommentare:

Cited Application: IHC

Negative Control: (ABIN7236730)

Blocking Peptide: (ABIN7236730)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu RTN4R

Target: RTN4R (Reticulon 4 Receptor (RTN4R))

Andere Bezeichnung: RTN4R (RTN4R Produkte)

Hintergrund: NogoR, Nogo-R, NgR, NgR1, Nogo-66 receptor, Reticulon 4 receptor, RTN4R,The Nogo receptor is a leucine-rich repeat (LRR) containing protein with a glycosylphosphatidylinositol (GPI) anchored C-terminus. The receptor was identified on the basis of its ability to bind with high affinity to Nogo-A a member of the reticulon family expressed in the cell membrane of oligodendrocytes.1Nogo-A attracted attention when it was demonstrated that it is a myelin protein capable of inhibiting axonal growth following nerve injury.2,3 Other myelin proteins also identified as axonal growth inhibitors are the myelin-associated glycoprotein (MAG) a sialic-dependent immunoglobulin-like family member lectin (SIGLEC) and the oligodendrocyte myelin glycoprotein (OmgP) which is a GPI-anchored membrane protein. Remarkably, despite their structural diversity, all three axonal growth inhibitors bind to the Nogo receptor with high affinity.4,5Since the Nogo receptor is a GPI-anchored protein it was expected that it would require another protein component to transduce the Nogo-A binding information into the responding neurons interior. Indeed, it was shown that the neurotrophin receptor p75 (p75NTR) interacts with Nogo receptor and a third protein termed LINGO-1 to mediate axonal growth inhibition signaling.6,7

Alternative names: Nogo Receptor, NogoR, Nogo-R, NgR, NgR1, Nogo-66 receptor, Reticulon 4 receptor, RTN4R

Gen-ID: 65078

NCBI Accession: NM_023004

UniProt: Q9BZR6

Pathways: Neurotrophin Signalübertragung