Regulating Synaptic Membrane Exocytosis 1 (RIMS1) (AA 1039-1054), (Intracellular) Antikörper

Produktnummer ABIN7043597

Produktdetails

Target: Regulating Synaptic Membrane Exocytosis 1 (RIMS1)

Bindungsspezifität: AA 1039-1054, Intracellular

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB)

Verwendungszweck: A Rabbit Polyclonal Antibody to RIM1

Spezifität: Intracellular

Kreuzreaktivität: Maus, Ratte

Homologie: human - 14,16 amino acid residues identical,Mouse - 15

Produktmerkmale: Anti-RIM1 Antibody is directed against an epitope of rat RIM1. Anti-RIM1 Antibody (ABIN7043597, ABIN7044528 and ABIN7044529) can be used in western blot analysis. It has been designed to recognize RIM1 from mouse, rat and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RAKRGRSAESLHMTSE, corresponding to amino acid residues 1039-1054 of rat RIM1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: (ABIN7236714)

Blocking Peptide: (ABIN7236714)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Antigendetails

Target: Regulating Synaptic Membrane Exocytosis 1 (RIMS1)

Andere Bezeichnung: RIMS1 (RIMS1 Produkte)

Hintergrund: Regulating synaptic membrane exocytosis 1, Rab-3-interacting molecule 1, RIMS1,RIM1 is a member of the RIM superfamily of proteins (Rim1-4). These genes express seven known RIM isoforms: RIM1α, 1β, RIM2α, 2β, 2γ, and RIM3γ and RIM4γ which serve as scaffolding proteins in presynaptic nerve terminals by interacting with several other protein components localized at the active zone, including Munc13, ELKS (or CAST), liprins and voltage-gated Ca2+ channels (VGCCs). RIM1 directly binds to the C-terminal regions of the α1 subunit of N- and P/Q-type calcium channels, and can also interact with the β auxiliary subunit of P/Q-type calcium channels1-3.RIM proteins were found to be required for normal Ca2+ triggering of exocytosis. This interaction with Ca2+ channel significantly slows channel inactivation resulting in increased Ca2+ influx during action potentials.Deficiency of RIM1 decreases presynaptic Ca2+ channel density. RIM proteins are involved in neurotransmitter release and contribute to long- and short-term synaptic plasticity.

Alternative names: RIM1, Regulating synaptic membrane exocytosis 1, Rab-3-interacting molecule 1, RIMS1

Gen-ID: 84556

NCBI Accession: NM_014989

UniProt: Q9JIR4

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity