QRFPR Antikörper (Extracellular)

Produktnummer ABIN7043594

Der Kaninchen Polyklonal Anti-QRFPR-Antikörper wurde für WB validiert. Er ist geeignet, QRFPR in Proben von Ratte zu detektieren.

Kurzübersicht für QRFPR Antikörper (Extracellular) (ABIN7043594)

Target: QRFPR (Pyroglutamylated RFamide Peptide Receptor (QRFPR))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser QRFPR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-QRFPR Antikörper

Bindungsspezifität: AA 18-32, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to QRFP Receptor

Spezifität: Extracellular, N-terminus

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse - identical, human - 13,17 amino acid residues identical

Produktmerkmale: Anti-QRFPR/GPR103 (extracellular) Antibody (ABIN7043594, ABIN7044421 and ABIN7044422) is a highly specific antibody directed against an epitope of the rat QRFP receptor. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and can potentially be used for detecting the receptor in living cells. It has been designed to recognize GPCR GPR103 from rat, mouse, and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HNLTREQFIHRYGLR, corresponding to amino acid residues 18-32 of rat QRFP receptor

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:500

Kommentare:

Negative Control: (ABIN7236703)

Blocking Peptide: (ABIN7236703)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu QRFPR

Target: QRFPR (Pyroglutamylated RFamide Peptide Receptor (QRFPR))

Andere Bezeichnung: QRFPR

Hintergrund: Pyroglutamylated RF-amide peptide receptor, Orexigenic neuropeptide QRFP receptor, G-protein coupled receptor 103, Peptide P518 receptor, SP9155, AQ27,QRFP is a 26-amino acid peptide (QRFP-26) that, widely distributed among vertebrates, is a member of the RF amide peptide family harboring the arginine-phenylalanine-amide (Arg-Phe-NH2) sequence motif in their carboxyl terminal. It is the cognate ligand of the human orphan receptor GPR103, a G protein-coupled receptor. Like other members of the super family, QRFP receptor has an extracellular N-terminal tail and intracellular C-terminus.Its QRFP N-terminus binds with high affinity to GPR103 and has the efficacy to inhibit cAMP synthesis. QRFP has been shown to affect locomotor activity, energy homeostasis, reproduction, bone formation, blood pressure, hormone regulation, nociception, and appetite stimulation.QRFP expressing neurons have shown to be expressed in various hypothalamic nuclei including the ventromedial hypothalamic nucleus, the lateral hypothalamic area, retrochiasmatic area and the arcuate nucleus1,2. The receptor is detected in the CNS and in peripheral tissues, notably in bone, heart, kidney, pancreas and testis.Increasing data suggest the ligand/receptor system plays an important role in the regulation of food intake and glucose homeostasis1.

Alternative names: QRFPR/GPR103, Pyroglutamylated RF-amide peptide receptor, Orexigenic neuropeptide QRFP receptor, G-protein coupled receptor 103, Peptide P518 receptor, SP9155, AQ27

Gen-ID: 310327

NCBI Accession: NM_198179

UniProt: P83858