Prostacyclin Receptor Antikörper (3rd Extracellular Loop)

Produktnummer ABIN7887012

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Prostacyclin Receptor in WB, FACS und LCI. Es zeigt Reaktivität gegenüber Proben von Ratte.

Kurzübersicht für Prostacyclin Receptor Antikörper (3rd Extracellular Loop) (ABIN7887012)

Target: Prostacyclin Receptor (PTGIR)

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Prostacyclin Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-Prostacyclin Receptor Antikörper

Bindungsspezifität: 3rd Extracellular Loop, AA 290-302

Verwendungszweck: A Rabbit Polyclonal Antibody to Prostacyclin Receptor (PTGIR)

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse - identical, human - 11,13 amino acid residues identical

Produktmerkmale: Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody is directed against an epitope of rat the PGI2 receptor. Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody (ABIN7043563, ABIN7045122 and ABIN7045123) can be used in western blot analysis. The antibody recognizes an extracellular epitope, ideal for detecting the receptor in living cells. It has been designed to recognize PGI2 receptor from human, mouse and rat samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)TQAIAPDSREMGD, corresponding to amino acid residues 290-302 of rat PTGIR

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200-1:400

FC: 5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7236620)

Blocking Peptide: (ABIN7236620)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu Prostacyclin Receptor

Target: Prostacyclin Receptor (PTGIR)

Andere Bezeichnung: PTGIR

Hintergrund:

Synonyms: Prostanoid IP receptor, Prostaglandin I2 receptor, PGI2 receptor

Description: Prostaglandins comprise a family of locally acting hormones, whose synthesis is initiated by the enzyme COX. This pathway is involved in a broad variety of pathological conditions such as cancer, inflammation and hypertension.Prostaglandin I2 receptor (PGI2 receptor) is G-coupled protein receptor (GPCR) with seven hydrophobic transmembrane segments and an extracellular NH2 terminus. Prostaglandin receptors belong to family A, group V of GPCRs. There is significant ligand-binding cross-reactivity between different prostaglandins and different receptors in the family. Within the PG family, receptors share roughly 20-30 % of their sequence and there are 34 identical residues across the family. Twenty of these residues are unique to PG receptors and are not shared by other GPCRs. The unique residues reside mostly in the transmembrane region and in the second extracellular loop region. Both of these regions are implicated in ligand binding. The PGI2 receptor signals primarily via stimulation of cAMP generation but through PIP2 as well despite a much higher agonist concentration required for PIP2 hydrolysis.PGI2 receptor mRNA is highly expressed in the human heart, aorta, kidney, liver and lung tissues and in mice thymus, heart and spleen1. Mice lacking PGI2 receptor have been shown to have a reduced inflammation response and a significant decrease in clinical arthritis. In addition, human patients with a dysfunctional PGI2 receptor variant exhibit an enhanced atherothrombotic phenotype with higher incidence of triple vessel coronary disease and cardiovascular events2.

Gen-ID: 292661

NCBI Accession: NM_000960

UniProt: P43253

Pathways: cAMP Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling