PRLHR Antikörper (3rd Intracellular Loop)

Produktnummer ABIN7887005

Der Kaninchen Polyklonal anti-PRLHR Antikörper (ABIN7887005) detektiert spezifisch PRLHR in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Ratte.

Kurzübersicht für PRLHR Antikörper (3rd Intracellular Loop) (ABIN7887005)

Target: PRLHR (Prolactin Releasing Hormone Receptor (PRLHR))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRLHR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-PRLHR Antikörper

Bindungsspezifität: 3rd Intracellular Loop, AA 247-260

Verwendungszweck: A Rabbit Polyclonal Antibody to Prolactin-Releasing Peptide Receptor

Kreuzreaktivität: Maus, Ratte

Homologie: Mouse - identical, human - 13,14 amino acid residues identical

Produktmerkmale: Anti-PrRP Receptor (GPR10) Antibody is directed against an epitope of the rat Prolactin-releasing peptide receptor. Anti-PrRP Receptor (GPR10) Antibody (ABIN7043562, ABIN7045116 and ABIN7045117) can be used in western blot analysis. It has been designed to recognize PrRPR from human, rat and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)RVSVKLRNRVVPGS, corresponding to amino acid residues 247-260 of rat PRLHR

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:1000

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7236617)

Blocking Peptide: (ABIN7236617)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu PRLHR

Target: PRLHR (Prolactin Releasing Hormone Receptor (PRLHR))

Andere Bezeichnung: PRLHR

Hintergrund:

Synonyms: Prolactin-releasing peptide receptor, PrRPR, Prolactin-releasing hormone receptor, PRLHR

Description: Prolactin-releasing peptide (PrRP) a bioactive peptide, was originally identified as an endogenous ligand of the orphan human G-protein coupled receptor, seven transmembrane domain receptor, GPR10 (hGR3, also called PrRP receptor). Like the majority of orphan receptors, PrRP receptor shows a low level of sequence homology with known GPCRs. Notwithstanding, a certain degree of homology has been reported to members of the neuropeptide Y (NPY) family of receptors. Neuropeptide Y is a 36- residue C-terminally amidated polypeptide hormone and neurotransmitter with the presence of an α-helix involving the 20 C-terminal residues, whereas the N-terminus part seems more flexible and is influenced by the medium environment1. It has been recently demonstrated that the PrRP receptor may modulate neurotransmission specifically at glutamatergic synapses through the interaction with the same PDZ domain proteins that interact with AMPA receptors2.Binding of PrRP to the receptor results in activation of extracellular signal-related kinase (ERK) in a mainly pertussis toxin sensitive manner, suggesting coupling to Gi/o proteins.PrRP receptor is expressed in the central nervous system with highest levels in the pituitary. Expression has also been detected in the cerebellum, brainstem, hypothalamus, thalamus, nucleus tractus solitaries, adrenal medulla and spinal cord in rat3.PrRP is implicated in the regulation of the hypothalamic-pituitary-adrenal axis, blood pressure and nociception4,5. Genetic studies in rodents indicate that lack of PrRP receptor leads to hyperphagia, obesity and dyslipidemia4. In humans, genetic variations in PrRP receptor are associated with lowered blood pressure5.

Gen-ID: 246075

NCBI Accession: NM_004248

UniProt: Q64121

Pathways: Feeding Behaviour