Nerve Growth Factor Antikörper (Pro-Domain)

Produktnummer ABIN7886725

Der Meerschweinchen Polyklonal anti-Nerve Growth Factor Antikörper wird verwendet zum Nachweis von Nerve Growth Factor in Proben von Ratte. Er wurde validiert für WB, IHC und IF.

Kurzübersicht für Nerve Growth Factor Antikörper (Pro-Domain) (ABIN7886725)

Target: Nerve Growth Factor (NGF)

Reaktivität: Ratte

Wirt: Meerschweinchen

Klonalität: Polyklonal

Konjugat: Dieser Nerve Growth Factor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-Nerve Growth Factor Antikörper

Bindungsspezifität: AA 84-104, Pro-Domain

Verwendungszweck: A Guinea Pig Polyclonal Antibody to proNGF

Kreuzreaktivität: Human, Maus, Ratte

Kreuzreaktivität (Details): The antibody is specific for proNGF, it does not crossreact with proBDNF, proNT-3 or mature NGF.

Homologie: Mouse - identical, human - 16,21 amino acid residues identical

Produktmerkmale: Guinea pig Anti-proNGF Antibody is directed against rat proNGF. Guinea pig Anti-proNGF Antibody can be used in western blot and immunohistochemistry applications. Anti-proNGF Antibody raised in guinea pig recognizes the same epitope as Anti-proNGF Antibody (ABIN7043556, ABIN7044751 and ABIN7044752) raised in rabbit. The antibody specifically reacts with proNGF and does not crossreact with proBDNF, pro or mature NGF. It has been designed to recognize proNGF from mouse, rat and human samples. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc. \nRelated products for neutralizing proNGF activity: \nMouse NGF/proNGF Neutralizing Antibody can be used in indirect ELISA and neutralization assays to block the biological effects of both NGF and proNGF.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)SPRVLFSTQPPPTSSDTLDLD, corresponding to amino acid residues 84-104 of rat NGF (precursor)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:400

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7236601)

Blocking Peptide: (ABIN7236601)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu Nerve Growth Factor

Target: Nerve Growth Factor (NGF)

Andere Bezeichnung: NGF

Hintergrund:

Synonyms: Nerve growth factor precursor

Description: Neurotrophins are synthesized as pro-forms that can be cleaved either intracellularly to release mature, secreted ligands, or extracellularly by various proteases such as plasmin, furin, PC1/3, PC7, and PACE 4.1,2,5 The immature precursor has a prodomain of 103 amino acids, which was thought to have a role in the folding and sorting of the mature NGF into the various secretion pathways. It was recently reported that proNGF, binds p75NTR receptor preferentially over TrkA, and this selective binding of proNGF to p75NTR leads to apoptotic death of cells that express both TrkA and p75NTR. However, mature NGF binds and activates both receptors, with resulting promotion of cell survival due to the TrkA-mediated survival signal overriding p75NTR -mediated apoptotic signal.3,4 Since pro- and mature neurotrophins seem to elicit opposite functional effects, by differential interactions with Trks and p75NTR receptors, extracellular cleavage represents a new way to control the synaptic functions of neurotrophins.It was demonstrated that proNGF from injured spinal cord extracts, is active and induce apoptosis among oligodendrocytes, and apoptosis can be blocked by a proNGF-specific antibody.6 Finally, proNGF was demonstrated as the predominant form in mouse, rat, and human brain tissue, thyroid, hippocampus, thus suggesting a role for proNGF in vivo.5,7,8

Gen-ID: 310738

NCBI Accession: NM_002506

UniProt: P25427

Pathways: Regulation of Cell Size