KCNK18 Antikörper (1st Extracellular Loop)

Produktnummer ABIN7886930

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch KCNK18 in WB, IHC, IF, IC und LCI. Es zeigt Reaktivität gegenüber Proben von Maus.

Kurzübersicht für KCNK18 Antikörper (1st Extracellular Loop) (ABIN7886930)

Target: KCNK18 (Potassium Channel Subfamily K Member 18 (KCNK18))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KCNK18 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Produktdetails anti-KCNK18 Antikörper

Bindungsspezifität: 1st Extracellular Loop, AA 62-75

Verwendungszweck: A Rabbit Polyclonal Antibody to KCNK18 Channel

Kreuzreaktivität: Maus, Ratte

Kreuzreaktivität (Details): Unlikely to recognize human samples.

Homologie: Rat - 13,14 amino acid residues identical

Produktmerkmale: Anti-KCNK18 (TRESK) (extracellular) Antibody is directed against an epitope of mouse KCNK18. Anti-KCNK18 (TRESK) (extracellular) Antibody (ABIN7043446, ABIN7045021 and ABIN7045022) can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize TRESK channel from mouse and rat samples. The antibody will unlikely recognize TRESK from human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)EAEENPELKKFLDD, corresponding to amino acid residues 62-75 of mouse KCNK18

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: 1:50

IHC: 1:100

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7236295)

Blocking Peptide: (ABIN7236295)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu KCNK18

Target: KCNK18 (Potassium Channel Subfamily K Member 18 (KCNK18))

Andere Bezeichnung: KCNK18

Hintergrund:

Synonyms: Potassium channel subfamily K member 18, TWIK-related spinal cord K+ channel, K2P18.1

Description: KCNK18 (also named TWIK-related spinal cord K+ channel, TRESK or K2P18.1) is a member of the 2-pore (2P) domain K+ channel family that currently includes 16 members. These channels show little time- or voltage-dependence and are considered to be "leak" or "background" K+ channels, thereby generating background currents which help set the membrane resting potential and control cell excitation.1The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C- termini. It has been proposed that the functional channel unit is a dimer.Different K2P family members are regulated by diverse physical and chemical stimuli including temperature, pH , mechanical stretch, inhalation anesthetics, signaling pathways (PKC and PKA), arachidonic acid, etc.KCNK18 is the only K2P channel, known so far, whose current is activated following Gαq-receptor coupled activation. The enhancement of KCNK18 current involves activation of calcineurin (calcium-calmodulin-dependent phosphatase 2B) following the rise in intracellular calcium that occurs subsequent to Gαq activation.2 In addition, KCNK18 is potently activated by clinical concentrations of volatile anesthetics.3KCNK18 expression in humans is largely restricted to the spinal cord although in rodents it has a broader expression pattern that includes brain, testis and spleen.KCNK18 represents the most important background K+ channel in dorsal root ganglion neurons and hence it has been postulated that it has an important role in acute and chronic pain as well as general anesthesia.

Gen-ID: 332396

NCBI Accession: NM_181840

UniProt: Q6VV64