KCNK18 Antikörper (Extracellular)

Produktnummer ABIN7043446

Produktdetails anti-KCNK18 Antikörper

Target: KCNK18 (Potassium Channel Subfamily K Member 18 (KCNK18))

Bindungsspezifität: AA 62-75, Extracellular

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KCNK18 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Verwendungszweck: A Rabbit Polyclonal Antibody to KCNK18 Channel

Spezifität: 1st extracellular loop

Kreuzreaktivität: Maus, Ratte

Kreuzreaktivität (Details): Unlikely to recognize human samples.

Homologie: Rat - 13,14 amino acid residues identical

Produktmerkmale: Anti-KCNK18 (TRESK) (extracellular) Antibody is directed against an epitope of mouse KCNK18. Anti-KCNK18 (TRESK) (extracellular) Antibody (ABIN7043446, ABIN7045021 and ABIN7045022) can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize TRESK channel from mouse and rat samples. The antibody will unlikely recognize TRESK from human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EAEENPELKKFLDD, corresponding to amino acid residues 62-75 of mouse KCNK18

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: (ABIN7236295)

Blocking Peptide: (ABIN7236295)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu KCNK18

Target: KCNK18 (Potassium Channel Subfamily K Member 18 (KCNK18))

Andere Bezeichnung: KCNK18 (KCNK18 Produkte)

Hintergrund: Potassium channel subfamily K member 18, TWIK-related spinal cord K+ channel, K2P18.1,KCNK18 (also named TWIK-related spinal cord K+ channel, TRESK or K2P18.1) is a member of the 2-pore (2P) domain K+ channel family that currently includes 16 members. These channels show little time- or voltage-dependence and are considered to be "leak" or "background" K+ channels, thereby generating background currents which help set the membrane resting potential and control cell excitation.1The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C- termini. It has been proposed that the functional channel unit is a dimer.Different K2P family members are regulated by diverse physical and chemical stimuli including temperature, pH , mechanical stretch, inhalation anesthetics, signaling pathways (PKC and PKA), arachidonic acid, etc.KCNK18 is the only K2P channel, known so far, whose current is activated following Gαq-receptor coupled activation. The enhancement of KCNK18 current involves activation of calcineurin (calcium-calmodulin-dependent phosphatase 2B) following the rise in intracellular calcium that occurs subsequent to Gαq activation.2 In addition, KCNK18 is potently activated by clinical concentrations of volatile anesthetics.3KCNK18 expression in humans is largely restricted to the spinal cord although in rodents it has a broader expression pattern that includes brain, testis and spleen.KCNK18 represents the most important background K+ channel in dorsal root ganglion neurons and hence it has been postulated that it has an important role in acute and chronic pain as well as general anesthesia.

Alternative names: KCNK18 (TRESK), Potassium channel subfamily K member 18, TWIK-related spinal cord K+ channel, K2P18.1

Gen-ID: 332396

NCBI Accession: NM_181840

UniProt: Q6VV64