Plexin A2 Antikörper (Extracellular)

Produktnummer ABIN7043437

Produktdetails anti-Plexin A2 Antikörper

Target: Plexin A2 (Plxna2)

Bindungsspezifität: AA 205-218, Extracellular

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Plexin A2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (IF), Live Cell Imaging (LCI)

Verwendungszweck: A Rabbit Polyclonal Antibody to Plexin-A2

Spezifität: Extracellular, N-terminus

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat,human - identical

Produktmerkmale: Anti-Plexin-A2 (extracellular) Antibody is directed against an epitope of mouse Plexin-A2. Anti-Plexin-A2 (extracellular) Antibody (ABIN7043437, ABIN7045130 and ABIN7045131)) can be used in western blot and immunohistrochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting PLXNA2 in living cells. It has been designed to recognize PLXNA2 from rat, mouse and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RDPESSAMLDYELH, corresponding to amino acid residues 205-218 of mouse PLXNA2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: BLP-PR082

Blocking Peptide: BLP-PR082

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu Plexin A2

Target: Plexin A2 (Plxna2)

Andere Bezeichnung: PLXNA2 (Plxna2 Produkte)

Hintergrund: PLXNA2, PLXN2, Semaphorin receptor OCT,The Plexin gene family is comprised of at least 9 members in 4 subfamilies. Plexins are widely expressed in neurons but have been also detected in epithelial cells1. Plexins closely interact with a large family of proteins called Semaphorins which control a variety of processes in the CNS including cell migration and axonal growth. Plexins and Semaphorins share a common feature of a conserved 500 amino acid "sema" domain2. The ectodomain of PLXNA2 is an N-terminal seven bladed β-propeller sema domain followed by a cysteine rich PSI (plexin-semaphorin-integrin) domain. PLXNA2 has four N-terminal domains and unlike semaphorins the sema domain of unliganded PLXNA2 does not homodimerize readily.Many of the functions of Class 3 semaphorins (Sema3A-Sema3G) are mediated by Plexin-A2 (PLXNA) and other A-plexins. Sema3 signaling depends on semaphorin interacting with one of the two Nrp co-receptors that associate with PLXNA to generate holoreceptors.PLXNA also interacts with semaphorin 6A (Sema6A). Before binding, the Sema6A ectodomain can be found in a 'face-to-face' homodimer arrangement, similar to the arrangement in Sema3, whereas PLXNA is in an unexpected 'head-on' homodimer arrangement. The structure of the Sema6A-PLXNA signaling complex is a 2:2 heterotetramer3.In prostate cancer TMPRSS:ERG gene fusion causes aberrant expression of the transcription factor ERG and the induction of cell migration and invasion. TMPRSS:ERG upregulates PLXNA expression and elevated PLXNA levels were found in prostate cancer cells. Levels of PLXNA were even higher in metastatic prostate cancer than in localized tumors suggesting PLXNA may play a role in the disease's development.

Alternative names: Plexin-A2, PLXNA2, PLXN2, Semaphorin receptor OCT

Gen-ID: 18845

NCBI Accession: NM_025179

UniProt: P70207