Plexin A1 Antikörper (Extracellular)

Produktnummer ABIN7043435

Produktdetails anti-Plexin A1 Antikörper

Target: Plexin A1 (PLXNA1)

Bindungsspezifität: AA 623-636, Extracellular

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Plexin A1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (IF), Live Cell Imaging (LCI)

Verwendungszweck: A Rabbit Polyclonal Antibody to Plexin-A1

Spezifität: Extracellular, N-terminus

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse,rat - 13,14 amino acid residues identical

Produktmerkmale: Anti-Plexin-A1 (extracellular) Antibody (ABIN7043435, ABIN7045128 and ABIN7045129)) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize PLXNA1 from mouse, rat and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)REVAPITRGQGDQR, corresponding to amino acid residues 623-636 of human PLXNA1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:600

Application Dilutions Western blot wb: 1:400

Kommentare:

Negative Control: BLP-PR081

Blocking Peptide: BLP-PR081

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu Plexin A1

Target: Plexin A1 (PLXNA1)

Andere Bezeichnung: PLXNA1 (PLXNA1 Produkte)

Hintergrund: PLXNA1, PLXN1, Semaphorin receptor NOV,The plexin family are transmembrane receptors, comprised four subfamilies: plexin-A, B, C, and D. In humans, three members of the plexin-A subfamily have been isolated: plexin-A1, A2, and A3. Plexin A1 is encoded by the PLXNA1 gene. These receptors are characterized by the presence of a conserved intracellular domain of about 600 amino acids known as the sex and plexins domain. This domain is strikingly conserved across the plexin family and consists of two blocks of high sequence conservation separated by a variable linker. The ectodomains of the plexin-A subfamily possess three repeats of the cysteine cluster/Met-related sequences.Plexins act as signal-transducing components containing transmembrane domain of six Gly residues constituting a GxGGGGG motif.Plexins act as functional receptors for members of the semaphorin family. Together they transmit repulsive axon guidance signals for semaphorins inside the axonal growth cone. The plexins act together with their co-receptors, neuropilins. Plexin-A1, in combination with neuropilins exhibits various functions in axon guidance, organogenesis, angiogenesis, tumorigenesis, and immune regulation1-3.Plexin-A1 also plays an important role in the developmental stages of chick heart by working as a receptor for one of the transmembrane semaphorins. In addition Plexin-A1 expressed in dendritic cells is involved in T-dendritic cell interactions in the immune system4.

Alternative names: Plexin-A1, PLXNA1, PLXN1, Semaphorin receptor NOV

Gen-ID: 5361

NCBI Accession: NM_032242

UniProt: Q9UIW2

Pathways: Smooth Muscle Cell Migration