P2RX3 Antikörper (Intracellular)

Produktnummer ABIN7043420

Der Kaninchen Polyklonal Anti-P2RX3-Antikörper wurde für WB, IHC, FACS, IF und IC validiert. Er ist geeignet, P2RX3 in Proben von Ratte zu detektieren.

Kurzübersicht für P2RX3 Antikörper (Intracellular) (ABIN7043420)

Target: P2RX3 (Purinergic Receptor P2x, Ligand-Gated Ion Channel, 3 (P2RX3))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser P2RX3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunochromatography (IC)

Produktdetails anti-P2RX3 Antikörper

Bindungsspezifität: AA 383-397, Intracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to P2X3 Receptor

Spezifität: Intracellular, C-terminus

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse - identical, human - 13,15 amino acid residues identical

Produktmerkmale: Anti-P2X3 Receptor Antibody (ABIN7043420, ABIN7045090 and ABIN7045091) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect flow cytometry, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize P2X3 receptor from rat, mouse, and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)VEKQSTDSGAYSIGH, corresponding to amino acid residues 383-397 of rat P2X3 receptor 

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Kommentare:

Cited Application: IP|IHC

Negative Control: (ABIN7236206)

Blocking Peptide: (ABIN7236206)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu P2RX3

Target: P2RX3 (Purinergic Receptor P2x, Ligand-Gated Ion Channel, 3 (P2RX3))

Andere Bezeichnung: P2RX3

Hintergrund: P2RX3, P2X purinoceptor 3, ATP Receptor,The P2X3 receptor belongs to the ligand-gated ion channel P2X receptor family, that consists of seven receptor subtypes named P2X1-P2X7 and is activated by extracellular ATP.1,2,3All P2X subunits, with the exception of P2X6, can assemble to form homomeric or heteromeric functional channels.4-5 The different P2X receptors show distinct expression patterns. P2X1-6 has been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system. The P2X3 receptor is highly expressed on nociceptive sensory neurons in dorsal root ganglia (DRG) as a homomer or as a heteromer (P2X3/P2X2). ATP released from damaged cells activates the P2X3 receptor to initiate nociceptive signals.6,7 Involvement of ATP in the mechanism of chronic pain has been also suggested.7,8 P2X3 receptor is now becoming a possible target for the development of pain therapeutics.

Alternative names: P2X3 Receptor, P2RX3, P2X purinoceptor 3

Gen-ID: 81739

NCBI Accession: NM_002559

UniProt: P49654