Otopetrin 1 Antikörper (Intracellular Loop)

Produktnummer ABIN7886501

Der Kaninchen Polyklonal anti-Otopetrin 1 Antikörper wird verwendet zum Nachweis von Otopetrin 1 in Proben von Maus. Er wurde validiert für WB und IHC.

Kurzübersicht für Otopetrin 1 Antikörper (Intracellular Loop) (ABIN7886501)

Target: Otopetrin 1 (OTOP1)

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Otopetrin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-Otopetrin 1 Antikörper

Bindungsspezifität: AA 374-388, Intracellular Loop

Verwendungszweck: A Rabbit Polyclonal Antibody to Otopetrin-1 Channel

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat,human - identical

Produktmerkmale: Anti-OTOP1 Antibody (ABIN7043417, ABIN7044503 and ABIN7044504) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize Otopetrin-1 from rat, mouse, and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)EKSLDESKNPARKLD, corresponding to amino acid residues 374 - 388 of mouse Otopetrin-1 

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7236195)

Blocking Peptide: (ABIN7236195)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu Otopetrin 1

Target: Otopetrin 1 (OTOP1)

Andere Bezeichnung: OTOP1

Hintergrund:

Synonyms: Proton Channel OTOP1, Otopetrin-1

Description: Otopetrin-1 (Otop-1) is a proton channel involved in sour taste recognition. It belongs to the Otopetrin proton channel family together with Otopetrins-2 and 31. Otop-1 is a dimeric structure, with each subunit forming 12 transmembrane helices divided into structurally similar N- and C-domains2.Taste recognition is very important because it enables to sample chemicals in the environment prior to digestion and hence, plays an important role in feeding and avoiding intoxication2. The sense of sour taste is caused by acids, and the first step in sour taste transduction is an influx of H+ ions through Otop-1 channel into the type three Taste Receptor Cells (TRCs). This leads to a decrease in intracellular pH and operation of a signaling cascade1-3.Otop-1 is specifically expressed in type three taste cells and exhibits the Zn2+ sensitivity that is a key feature observed in the proton current of sour taste cells4.Otop-1 also has a role in regulating intracellular Ca2+ levels required for the formation of otoconia in the vertebrate inner ear. Otoconia are complex calcium carbonate biominerals required for the sensation of gravity5.

Gen-ID: 21906

NCBI Accession: NM_177998

UniProt: Q80VM9