SLC22A3 Antikörper (Extracellular)

Produktnummer ABIN7043416

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SLC22A3 in WB, IHC und IF. Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für SLC22A3 Antikörper (Extracellular) (ABIN7043416)

Target: SLC22A3 (OCT3) (Organic Cation Transporter 3 (OCT3))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SLC22A3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-SLC22A3 Antikörper

Bindungsspezifität: AA 83-96, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to Organic Cation Transporter 3

Spezifität: 1st extracellular loop

Kreuzreaktivität: Maus, Ratte

Kreuzreaktivität (Details): Not recommended for human samples.

Homologie: Rat,mouse - 13,14 amino acid residues identical

Produktmerkmale: Anti-SLC22A3 (extracellular) Antibody (ABIN7043416, ABIN7044223 and ABIN7044224) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Organic cation transporter 3 from rat and mouse samples. The antibody is unlikely to recognize the protein from human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)ERRGQGH(S)HRYLLE, corresponding to amino acid residues 83-96 of rat OCT3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: (ABIN7236191)

Blocking Peptide: (ABIN7236191)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu SLC22A3

Target: SLC22A3 (OCT3) (Organic Cation Transporter 3 (OCT3))

Andere Bezeichnung: SLC22A3

Hintergrund: OCT3, Organic cation transporter 3,Organic cation transporter 3 (SLC22A3) belongs to the SLC22A gene family. Organic cation transporters (OCTs) are membrane transport proteins involved in many metabolic processes and detoxification. The transporters are responsible for the uptake and intracellular inactivation of numerous endogenous and exogenous substrates including norepinephrine, epinephrine, dopamine, histamine and serotonin. They are also responsible for the uptake of a variety of anticancer drugs1,2. OCT structure is suggested to have 12 transmembrane domains and a large hydrophobic cleft capable of accommodating a wide variety of chemical species3.In humans, OCT3 is expressed in many tissues, abundantly in the placenta, heart, liver and skeletal muscle. It is also expressed in astrocytes, intestinal epithelium neurons, glia, ependymal cells, and blood-cerebrospinal fluid barrier in choroid plexus epithelial cells. The protein is also widely distributed in the brain. OCT expression is detected in several cancer cell lines and tumor tissue samples1-3.

Alternative names: SLC22A3, OCT3, Organic cation transporter 3

Gen-ID: 29504

NCBI Accession: NM_021977

UniProt: O88446