OPRK1 Antikörper (Extracellular)

Produktnummer ABIN7043402

Der Kaninchen Polyklonal Anti-OPRK1-Antikörper wurde für WB, IHC, FACS und LCI validiert. Er ist geeignet, OPRK1 in Proben von Ratte zu detektieren.

Kurzübersicht für OPRK1 Antikörper (Extracellular) (ABIN7043402)

Target: OPRK1 (Opioid Receptor, kappa 1 (OPRK1))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser OPRK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-OPRK1 Antikörper

Bindungsspezifität: AA 39-55, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to ?-Opioid Receptor (OPRK1)

Spezifität: Extracellular, N-terminus

Kreuzreaktivität: Maus, Ratte

Homologie: human - 15,17 amino acid residues identical,Mouse -16

Produktmerkmale: Anti-κ-Opioid Receptor (OPRK1) (extracellular) Antibody is directed against an epitope of the rat κ-opioid receptor. Anti-κ-Opioid Receptor (OPRK1) (extracellular) Antibody (ABIN7043402, ABIN7044889 and ABIN7044890) can be used in western blot analysis. The antibody recognizes an extracellular epitope and thus is ideal for detecting the receptor in living cells. The antibody was designed to recognize κ-opioid receptor from mouse, rat and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)NGSVGSEDQQLEPAHIS, corresponding to amino acid residues 39-55 of rat kappa-Opioid receptor

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Kommentare:

Cited Application: IHC

Negative Control: (ABIN7236171)

Blocking Peptide: (ABIN7236171)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu OPRK1

Target: OPRK1 (Opioid Receptor, kappa 1 (OPRK1))

Andere Bezeichnung: OPRK1

Hintergrund: Kappa-type opioid receptor, KOR-1, Opiate receptor kappa 1,Endogenous opiates such as endorphins, endomorphins, and enkephalins, as well as opiate drugs (including morphine) exert their effects by binding to opioid receptors. Three "classic" types of opioid receptors have been identified: mu (μ)-opioid (MOP) receptor, delta (δ)-opioid (DOP) receptor, and kappa (κ)-opioid (KOP) receptor1. Recently, the nociceptin/orphanin FQ (N/OFQ) peptide (NOP) receptor was also described. Despite its significant sequence homology, its pharmacological profile differs greatly from those of the classic μ, δ, and κ receptors2.The opioid receptors belong to the G protein-coupled receptor (GPCR) superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop important for binding G proteins1.All three classic opioid receptors mediate opioid-induced analgesia. Supraspinal analgesia is mainly mediated by the μ-opioid receptor, whereas μ-, δ-, and κ-receptors participate in the control of pain at the spinal level3. The opioid receptors also mediate the mood-altering properties of opioids4.κ-Opioid Receptor is activated by the endogenous peptide dynorphin. Malfunction of their signaling may be involved in epilepsy, addiction, depression, schizophrenia, and chronic pain5.κ-Opioid Receptor is localized to a number of regions in the brain, as well as the spinal cord, the enteric nervous system and the digestive system5.

Alternative names: Kappa-Opioid Receptor (OPRK1), Kappa-type opioid receptor, KOR-1, Opiate receptor kappa 1

Gen-ID: 29335

NCBI Accession: NM_000912

UniProt: P34975