NIPAL4 Antikörper (Extracellular)

Produktnummer ABIN7043394

Dieses Anti-NIPAL4-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von NIPAL4 in WB und FACS. Geeignet für Human.

Kurzübersicht für NIPAL4 Antikörper (Extracellular) (ABIN7043394)

Target: NIPAL4 (NIPA-Like Domain Containing 4 (NIPAL4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NIPAL4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Produktdetails anti-NIPAL4 Antikörper

Bindungsspezifität: AA 242-256, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to Magnesium Transporter NIPA4 

Spezifität: 2nd extracellular loop

Kreuzreaktivität: Human, Maus, Ratte

Homologie: rat - 12,15 amino acid residues identical,Mouse - 13

Produktmerkmale: Anti-NIPAL4 (extracellular) Antibody is directed against an epitope of human magnesium transporter NIPA4. Anti-NIPAL4 (extracellular) Antibody (ABIN7043394, ABIN7044847 and ABIN7044848) can be used in western blot and indirect flow cytometry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the transporter in living cells. It has been designed to recognize NIPAL4 from mouse, rat and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EKVTTIMEMASKMKD, corresponding to amino acid residues 242-256 of human magnesium transporter NIPA4

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400

Kommentare:

Negative Control: (ABIN7236151)

Blocking Peptide: (ABIN7236151)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu NIPAL4

Target: NIPAL4 (NIPA-Like Domain Containing 4 (NIPAL4))

Andere Bezeichnung: NIPAL4

Hintergrund: Magnesium transporter NIPA4, NIPA-like protein 4, NIPA-like domain containing 4, Ichthyin, ICHYN, ICHN, Non-imprinted in Prader-Willi/Angelman syndrome region protein 4,The NIPA family is currently comprised of 4 members- NIPA1-4. These members have a molecular similarity of 40 % . NIPA proteins are integral membrane proteins which function as Mg2+ transporters.The NIPA4 gene in mice is located on chromosome 11B1.1 and the human corresponding gene, hIchthyin is located on chromosome 5q33.3. Both genes share an 85 % amino acid similarity. Interestingly, NIPA2-4 show greater common identity in their ancestral invertebrate polypeptides than they do to NIPA1 suggesting different physiological roles. NIPA4 mediates Mg2+ uptake with a Km of 0.36 mM but unlike NIPA2, which is selective for Mg2+, it has been found to transport other cations as well.Mutations in the hIchthyin gene provide the basis of one form of autosomal-recessive congenital ichthyosis (ARCI). ARCI is a heterogenous group of disorders with abnormal differentiation and desquamation of the epidermis, leading to widespread scaling and erythema of the skin. To this day six homozygous mutations have been found, including one nonsense and five missense mutations, in a large number of consanguineous families presenting with ichthyosis. The function of ichthyin in skin differentiation is not fully understood but it has been suggested that because lipid dysfunctions are involved with the other ARCI disorders ichthyin might perform as a membrane receptor for trioxilins of the hepoxilin pathway.Although some NIPA proteins have been implicated as candidates for prader-willi syndrome their involvement remains unclear. Currently there is no evidence that NIPA4 is directly involved in the many complexities of the syndrome1.

Alternative names: NIPAL4, Magnesium transporter NIPA4, NIPA-like protein 4, NIPA-like domain containing 4, Ichthyin, ICHYN, ICHN, Non-imprinted in Prader-Willi/Angelman syndrome region protein 4

Gen-ID: 348938

NCBI Accession: NM_001099287

UniProt: Q0D2K0