NTSR1 Antikörper (Extracellular)

Produktnummer ABIN7043383

Produktdetails anti-NTSR1 Antikörper

Target: NTSR1 (Neurotensin Receptor 1 (High Affinity) (NTSR1))

Bindungsspezifität: AA 209-224, Extracellular

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NTSR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunofluorescence (Cultured Cells) (IF (cc)), Immunochromatography (IC), Live Cell Imaging (LCI)

Verwendungszweck: A Rabbit Polyclonal Antibody to Neurotensin Receptor 1

Spezifität: 2nd extracellular loop

Kreuzreaktivität: Human, Ratte

Homologie: rat - 12,16 amino acid residues identical,Mouse - 14

Produktmerkmale: Anti-Neurotensin Receptor 1 (extracellular) Antibody (ABIN7043383, ABIN7044767 and ABIN7044768)) is a highly specific antibody directed against an epitope of the human NTSR1. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and flow cytometry applications. It has been designed to recognize NTSR1 from human, mouse, and rat samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: EQNRSADGQHAGGLVC corresponding to amino acid residues 209-224 of human NTSR1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: BLP-NT015

Blocking Peptide: BLP-NT015

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu NTSR1

Target: NTSR1 (Neurotensin Receptor 1 (High Affinity) (NTSR1))

Andere Bezeichnung: NTSR1 (NTSR1 Produkte)

Hintergrund: NTR1, NTSR1, NTS1, High-affinity levocabastine-insensitive neurotensin receptor, NTRH, NTRR,Neurotensin receptor 1 (NTR1) is one of three receptors that mediate the effects of the tridecapeptide neurotensin. Neurotensin is synthesized and secreted from neurons in the central neural system (CNS) and from endocrine cells in the gastrointestinal tract.NTR1 and NTR2 belong to the 7-transmembrane domain, G-protein coupled receptor (GPCR) superfamily while the third neurotensin receptor NTR3 (also called Sortilin) is a type I membrane protein with a large extracellular domain. Both NTR1 and NTR3 bind neurotensin with high affinity while NTR2 binds it with low affinity.1NTR1 signals preferentially through Gaq, resulting in the activation phopholipase C and intracellular Ca2+ mobilization, although coupling with Gi/o and to Gs has been observed in some expression systems.NTR1 is mainly expressed in the brain and gastrointestinal tract. In the brain, NTR1 mediates body temperature regulation, feeding behavior and locomotion regulation. One of the best studied roles of NTR1 is the regulation of dopamine release suggesting that NTR1 can be a suitable target for the development of a new class of anti-psychotics.2 In the periphery, NTR1 expression has been linked to the control of intestine motility and in the stimulation of growth of the intestine mucosa in both physiological and pathological conditions. NTR1 has been shown to be upregulated in several human cancers where it stimulates tumor growth in response to locally produced neurotensin peptide.3

Alternative names: Neurotensin Receptor 1, NTR1, NTSR1, NTS1, High-affinity levocabastine-insensitive neurotensin receptor, NTRH, NTRR

Gen-ID: 4923

NCBI Accession: NM_002531

UniProt: P30989