NETO2 Antikörper (Extracellular, N-Term)

Produktnummer ABIN7043377

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch NETO2 in WB, IHC, FACS, IF und LCI. Es zeigt Reaktivität gegenüber Proben von Ratte.

Kurzübersicht für NETO2 Antikörper (Extracellular, N-Term) (ABIN7043377)

Target: NETO2 (Neuropilin (NRP) and Tolloid (TLL)-Like 2 (NETO2))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NETO2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Live Cell Imaging (LCI)

Produktdetails anti-NETO2 Antikörper

Bindungsspezifität: AA 189-202, Extracellular, N-Term

Verwendungszweck: A Rabbit Polyclonal Antibody to NETO2

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse,human - identical

Produktmerkmale: Anti-NETO2 (extracellular) Antibody (ABIN7043377, ABIN7044386 and ABIN7044387) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NETO2 from human, rat, and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)RSSQVEQEEKTKPG, corresponding to amino acid residues 189-202 of rat NETO2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7236110)

Blocking Peptide: (ABIN7236110)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu NETO2

Target: NETO2 (Neuropilin (NRP) and Tolloid (TLL)-Like 2 (NETO2))

Andere Bezeichnung: NETO2

Hintergrund:

Synonyms: Neuropilin and tolloid-like protein 2, Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 2, BTCL2

Description: NETO proteins are auxiliary subunits that play a crucial role in the modulation of the biophysical properties of Kainate-type glutamate receptors (KARs). KARs are activated to modulate synaptic transmission, network excitability and synaptogenesis. NETO proteins modulate their activity both in vivo and in vitro mostly by regulating the surface expression and synaptic targeting of KARs in neurons.NETO protein expression has been characterized in the adult brain. NETO1 and NETO2 are highly homologous but have different expression patterns and distinct functional effects. High NETO2 expression levels can be found throughout the mammalian brain1,2.The NETO2 structure reveals a single-pass transmembrane protein that contains an extracellular region with two CUB domains that mediates interactions with KAR subunits. The C-terminus is important for modifying and regulating the synaptic abundance of KARs. The intracellular region of NETO2 can be phosphorylated by various kinases in vitro,which regulates synaptic targeting of KARs3.

Gen-ID: 307757

NCBI Accession: NM_018092

UniProt: C6K2K4